中文摘要：本文展示了一种新的基于磁珠的酶联免疫吸附测定法(MB-ELISA)，用于检测玉米赤霉烯酮(ZEN)。研究发现，用MB-ELISA分析天然玉米样品，其结果与常规直接竞争ELISA的结果完全一致。
外文摘要：Here we demonstrate a novel magnetic bead-based enzyme-linked immunosorbent assay (MB-ELISA) for zearalenone (ZEN) detection. Firstly, an anti-ZEN monoclonal antibody (mAb) was prepared by hybridoma technique, and immobilized on carboxyl modified MBs to obtain mAb-MBs. In addition, the biotinylated ZEN-BSA was labelled by streptavidin-HRP for use as competitor. Based on the mAb-MBs and streptavidin-HRP labelled ZEN-BSA, a MB-ELISA which contains only one 20 min antigen-antibody reaction step and takes no more than 45 min for dozens of samples analysis was developed. The half maximal inhibitory concentration (IC50) and limit of detection (LOD) of the MB-ELISA are 1.78 ng/mL and 0.13 ng/mL, respectively. And the MB-ELISA working range for corn samples analysis is from 5.0 μg/kg to 255.2 μg/kg. The recoveries for ZEN spiked corn samples ranged from 82.3 to 110.5% with coefficient of variation (CV) under 8.9%. For natural corn samples analysis, the results of MB-ELISA showed good agreement with the results of conventional direct competitive ELISA (R2 = 0.9742).
外文关键词：Zearalenone；Monoclonal antibody；Magnetic beads；ELISA；Rapid
作者：Zhao, FC；Shen, Q；Wang, HM；Han, X；Yang, ZY
作者单位：Shandong Agr Univ
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2017
出版刊次：9
点击下载：开发一种基于磁珠的快速免疫分析法用于灵敏地检测玉米赤霉烯酮