中文摘要：本研究开发并彻底验证了一种灵敏测定巴西坚果中黄曲霉毒素B1、B2、G1和G2的遵守欧盟认可的标准观测参数的方法。研究表明这是一种监测与研究巴西坚果中黄曲霉毒素的可靠、灵敏且安全的分析法。
外文摘要：A sensitive method for determination of aflatoxins B1, B2, G1 and G2 in Brazil nut has been developed and thoroughly validated observing parameters of compliance criteria recognized by the European Union. Samples were extracted with methanol, cleaned-up with immunoaffinity columns and analyzed and quantified by high-performance liquid chromatography with fluorescence detection and post-column derivatization. High linearity was obtained over a dynamic range of 0.2–7.0 μg/kg for each aflatoxin. Recoveries varied from 90.6 to 102%, with reproducibility standard deviations lower than 9.5%. Limits of quantification were 0.18 μg/kg for B1, G1 and G2 and 0.10 μg/kg for B2. Expanded measurement uncertainties at 5.0 μg/kg and 20 μg/kg contamination levels for B1 and total aflatoxin, respectively, were ±0.789 μg/kg and ±2.99 μg/kg. Decision limits (CCα) and detection capabilities (CCβ), robustness and trueness were also evaluated. The result is a robust, sensitive and safe analytical method for surveillance and research of aflatoxins in Brazil nuts.
外文关键词：Brazil nut；Validation；Quantification；Aflatoxins；HPLC；Imunoaffinity column clean-up
作者：Campos, WEO；Rosas, LB；Neto, AP；Mello, RA；Vasconcelos, AA
作者单位：Fed Univ Para
期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS
期刊影响因子：2.78
出版年份：2017
出版刊次：7
点击下载：用HPLC-FLD扩展验证一种灵敏而可靠的同时量化巴西坚果中的黄曲霉毒素B1、B2、G1和G2的方法