中文摘要：在饲料供应链中，镰刀菌及霉菌毒素伏马菌素B1频繁地污染畜禽饲料。本研究评估了在辐照和非辐照的畜禽饲料混合物中产伏马菌素B1的镰刀菌的发生率，以及γ射线(2.5、5.0、7.5和10.002kGy)对尽量减少人工接种的饲料中镰刀菌生长及伏马菌素B1生物合成的效率。结果表明超过7.502kGy的γ射线能有效阻止混合饲料中真菌的生长并尽量减少动物和人类生活霉菌毒素暴露的潜在风险。也很有必要维持饲料的正常储存系统，直到喂食。
外文摘要：Contamination of animal and poultry feeds by Fusarium and the mycotoxin Fumonisin B1 is frequent in the feed supply chain. The present study evaluated the prevalence of fumonisin B1 producing Fusarium among irradiated and non-irradiated animal and poultry feed mixtures. Further, the efficiency of γ-rays (2.5, 5.0, 7.5, and 10.002kGy) to minimize Fusarium growth and biosynthesis of fumonisin B1 in artificially inoculated feed was evaluated. A total of 108 feed samples were collected in which 45.37% of feed mixtures were contaminated with Fusarium species. Among the contaminated samples, the frequency levels ofF. verticillioidesandF. proliferatumwere 42.59 and 24.07%, respectively. Out of the 98Fusariumisolates from feed samples, 84.7% ofF. verticillioidesand 64.28% ofF. proliferatumwere positive for FUM1 set of primers. Fumonisin B1 biosynthesis by the FUM1 positive isolates in feed was confirmed by LC/MS which recorded 0.1–450208g/g of feed. Fungal growth and viable count ofFusariumin PDA medium and feed decreased with increasing irradiation dosage. Interestingly, fumonisin content was 110208g/g of feed in 2.502kGy irradiated sample as compared to 50208g/g of feed in non-irradiated control. Ionizing radiation at 7.502kGy was found lethal for fungal growth and fumonisin production. Our findings suggest that γ-radiation above 7.502kGy effectively prevented fungal growth in feed mixtures and minimized the exposure of animal and human life to the potential risk of mycotoxin. Also it is necessary to maintain proper storage system for feeds until consumption.
外文关键词：Fusarium, Fumonisin B1；Animal feed；Poultry feed；gamma irradiation
作者：Deepthi, BV；Gnanaprakash, AP；Sreenivasa, MY
作者单位：Univ Mysore
期刊名称：3 BIOTECH
期刊影响因子：0.992
出版年份：2017
出版刊次：4
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