中文摘要：白僵菌会感染多种害虫，以能够产生杀虫物质，如非核糖体肽(NRPs)白僵菌素及类白僵菌素而闻名。然而，白僵菌中大多数NRPs及其生物学作用仍未被发现。为了鉴定可能有助于发病机制的NRPs，将预测的白僵菌 ARSEF 2860的21个NRP合成酶（NRPS）或NRPS样蛋白分为三个功能组：基础代谢（7 NRPSs），致病性（12 NRPSs）和未知功能（2 NRPSs）。鉴于这些NRPSs生物合成的代谢物仍有待确定，本研究结果突显了NRPSome在真菌发病机制中的重要性，并将它作为未来基因组挖掘项目的指南，以发现真菌基因组中功能基本化和结构多样化的NRPs。
外文摘要： Beauveria bassiana infects a number of pest species and is known to produce insecticidal substances, such as the nonribosomal peptides (NRPs) beauvericin and bassianolide. However, most NRPs and their biological roles in B. bassiana remain undiscovered. To identify NRPs that potentially contribute to pathogenesis, the 21 predicted NRP synthetases (NRPSs) or NRPS-like proteins of B. bassiana ARSEF 2860 were primarily ranked into three functional groups: basic metabolism (7 NRPSs), pathogenicity (12 NRPSs), and unknown function (2 NRPSs). Based on the transcript levels during in vivo growth on diamondback moth (Plutella xylostella (Linnaeus)), half of the Group II NRPSs were likely to be involved in infection. Given that the metabolites biosynthesized by these NRPSs remain to be determined, our result underlines the importance of the NRPSome in fungal pathogenesis, and will serve as a guide for future genomic mining projects to discover functionally essential and structurally diverse NRPs in fungal genomes.
外文关键词：Nonribosomal peptide synthetase；secondary metabolites；entomopathogen
作者：Liu,H；Xie,LA；Wang,J；Guo,QN；Yang,SN；Liang,P；Wang,CS； Lin,M；Xu,YQ；Zhang,LW
作者单位：Chinese Acad Agr Sci
期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
期刊影响因子：1.685
出版年份：2017
出版刊次：3
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