中文摘要：存在于大豆食品和膳食补充剂中的染料木黄酮以其雌激素潜力而闻名。除了植物雌激素之外，食物还可能含有具有雌激素性质的霉菌毒素如玉米赤霉烯酮或交链孢酚，从而引发了这些外源雌激素的潜在组合作用的问题。本文在人子宫内膜腺癌石川细胞系中研究了染料木黄酮与玉米赤霉烯酮或交链孢酚的组合雌激素作用，该作用会影响碱性磷酸酶（AlP）活性的表达。研究证明了植物和真菌毒素雌激素的混合效应可能给消费者带来意想不到的风险。强调了在未来的风险评估中考虑组合效应的必要性。应用的体外研究设计是一种经济高效的筛选方法，以发现雌激素的交互作用作为优先风险评估的基本决策工具。
外文摘要：Scope The isoflavone genistein, present in soy-based food and dietary supplements, is known for its estrogenic potential. In addition to phytoestrogens, food may also contain mycotoxins with estrogenic properties like zearalenone or alternariol, raising the question on potential combinatory effects of these xenoestrogens. Methods and resultsCombinatory estrogenic effects of genistein with zearalenone or alternariol were studied in the human endometrial adenocarcinoma cell line Ishikawa as expression of alkaline phosphatase (AlP) activity. Combinations of genistein with either zearalenone or alternariol, showed synergism and antagonism in the AlP assay, depending on the combination ratios and the concentration range. For combinations of zearalenone with genistein synergistic effects dominated. ConclusionWe demonstrate that mixture effects of phyto- and mycoestrogens potentially pose unexpected risks to consumers. Our study highlights the necessity of according considerations regarding combinatory effects in future risk assessment. The applied in vitro study design represents a cost-efficient screening method to discover interactive effects of estrogens as a basic decision tool for priority risk assessment.
外文关键词：Chemical mixtures；Food contaminants；Food safety；Polyphenol；Xenoestrogen
作者：Vejdovszky,K；Schmidt,V；Warth,B；Marko,D
作者单位：Univ Vienna
期刊名称：MOLECULAR NUTRITION & FOOD RESEARCH
期刊影响因子：4.551
出版年份：2017
出版刊次：3
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