中文摘要：赤霉病（FHB）是由镰刀菌引起的全球小麦（Triticum aestivum）毁灭性的病害。禾谷镰刀菌产生的很多种毒素比如脱氧雪腐镰刀烯醇（DON），对植物和动物的健康有负面影响，并造成粮食产量和质量的明显降低。抗性品种是控制这种疾病的唯一有效途径。然而，防御抵抗的分子机制仍不清楚。为了清楚地了解，本研究用定量逆转录PCR（qRT-PCR）分别在接种后6、12、24、36、48、72和144h对Sumai3号小麦品种（抗性）和Caledonia（易感）进行了一些消减差异表达的宿主基因和一些真菌表达基因的转录谱分析。本研究记录了八个上调的宿主克隆基因模式与小麦数据库显著匹配，七个真菌克隆与禾谷镰刀菌序列显著匹配。
外文摘要：Fusarium head blight (FHB) caused by Fusarium species is a devastating disease of wheat (Triticum aestivum) worldwide. Mycotoxins such as deoxynivalenol (DON) produced by Fusarium graminearum negatively affect plant and animal health, and cause significant reductions in grain quantity and quality. Resistant cultivars are the only effective way to control this disease; however, the molecular mechanisms of defense resistance are still unclear. To get a clear insight, transcriptional profiling of some subtracted differentially expressed host genes and some fungal expressed genes were carried out for the Sumai 3 wheat cultivar (resistant) and Caledonia (susceptible) at 6, 12, 24, 36, 48, 72 and 144 h post inoculation (hpi) using quantitative reverse transcription PCR (qRT-PCR). We document in this study the gene patterns of eight up-regulated host clones with significant matches to T. aestivum database and seven fungal clones with significant matches to F. graminearum sequences.
外文关键词：Fusarium graminearum； Wheat； Suppression subtractive hybridization； Gene expression； qRT-PCR
作者：AlTaweel, K；Amarasinghe, CC；Brule-Babel, AL；Fernando, WGD
作者单位：Univ Manitoba
期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY
期刊影响因子：1.494
出版年份：2017
出版刊次：3
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