中文摘要：本文报道了一种灵敏的检测食品中黄曲霉毒素B1（AFB1）的比色免疫测定法。研究表明该方法的精度（用RSD表示）为±9.7%。通过分析天然污染和加标的食品样品验证其准度，结果与ELISA结果吻合良好。
外文摘要：This work reports on a sensitive colorimetric immunoassay for aflatoxin B1 (AFB1) in foodstuff. The reagent L-ascorbic acid 2-phosphate (AAP) is added to the system, and alkaline phosphatase (ALP) hydrolyzes AAP under formation of ascorbic acid and phosphate. The ascorbic acid produced reduces chloroauric acid to form zero-valent gold (in the form of nanoparticles). Hence, Au(III) is no longer available to oxidize 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) to form a green product. Rather, the solution remains colorless. By using ALP-labelled monoclonal anti-AFB1 antibody, a competitive enzyme-label immunoassay was developed for AFB1 in a microplate coated with the AFB1-BSA conjugate. Under optimal conditions, the absorbance of the solution at 415 nm increases linearly with increasing AFB1 concentration in range from 10 pg·mL−1 to 100 ng·mL−1 (while the color gradually turns to green), and the detection limit is 7.8 pg·mL−1. The precision of the method (expressed as RSD) is ±9.7%. The accuracy was validated by analyzing both naturally contaminated and spiked food samples, and the results matched the results obtained by ELISA very well.
外文关键词：Colorimetric immunoassay；Single-shot assay；UV-vis adsorption spectroscopy；Cuvette；Microtiter plate；Oxidation-reduction reaction；Enzymatic catalytic reaction；Naturally contaminated/spiked food sample
作者：Tang, Y；Lai, WQ；Zhang, J；Tang, DP
作者单位：Chongqing Univ Arts & Sci
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2017
出版刊次：7
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