中文摘要：在所有污染食品的霉菌毒素中，黄曲霉毒素，特别是黄曲霉毒素B1（AFB1），毒性最强，损失最重。绿色农业一直致力于替代杀菌剂并且开发自然防御策略，尽量减少这些有毒真菌代谢物污染作物。本研究证明了一种药用植物姜味草属（即牛膝草）水提物可以完全抑制黄曲霉产生黄曲霉毒素而不会减少真菌生长。研究显示牛膝草在转录组学水平上抑制AFB1的合成。该水提物是一种控制AFB1污染的有前景且纯天然的溶液。
外文摘要：Of all the food-contaminating mycotoxins, aflatoxins, and most notably aflatoxin B-1 (AFB(1)), are found to be the most toxic and economically costly. Green farming is striving to replace fungicides and develop natural preventive strategies to minimize crop contamination by these toxic fungal metabolites. In this study, we demonstrated that an aqueous extract of the medicinal plant Micromeria graeca known as hyssop completely inhibits aflatoxin production by Aspergillus flavus without reducing fungal growth. The molecular inhibitory mechanism was explored by analyzing the expression of 61 genes, including 27 aflatoxin biosynthesis cluster genes and 34 secondary metabolism regulatory genes. This analysis revealed a three-fold down-regulation of aflR and aflS encoding the two internal cluster co-activators, resulting in a drastic repression of all aflatoxin biosynthesis genes. Hyssop also targeted fifteen regulatory genes, including veA and mtfA, two major global-regulating transcription factors. The effect of this extract is also linked to a transcriptomic variation of several genes required for the response to oxidative stress such as msnA, srrA, catA, cat2, sod1, mnsod, and stuA. In conclusion, hyssop inhibits AFB(1) synthesis at the transcriptomic level. This aqueous extract is a promising natural-based solution to control AFB(1) contamination.
外文关键词：Aflatoxin B-1；Aspergillus flavus；hyssop；inhibition；oxidative stress
作者：El Khoury, Rhoda；Caceres, Isaura；Puel, Olivier
作者单位：Universite de Toulouse
期刊名称：TOXINS
期刊影响因子：3.029
出版年份：2017
出版刊次：3
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