中文摘要：在食品或者饲料管控中，霉菌毒素的检测通常一次完成一种分析物。本文提出的方法用于同步检测四种霉菌毒素时，通过利用乙酸乙酯替代乙腈、放弃样品提取净化、最小化溶剂的消耗来减少化学废料的产生，从而使霉菌毒素分析对环境更加友好。研究证明本方法比现有方法更绿色更快捷。
外文摘要：In food/feed control, mycotoxin analysis is often still performed one analyte at a time. Here a method is presented which aims at making mycotoxin analysis environmentally friendlier through replacing acetonitrile by ethyl acetate and reducing chemical waste production by analyzing four mycotoxins together, forgoing sample extract clean-up, and minimizing solvent consumption. For this, 2 g of test material were suspended in 8 mL water and 16 mL ethyl acetate were added. Extraction was accelerated through sonication for 30 min and subsequent addition of 8 g sodium sulfate. After centrifugation, 500 mu L supernatant were spiked with isotopologues, dried down, reconstituted in mobile phase, and measured with LC-MS. The method was validated in-house and through a collaborative study and the performance was fit-for-purpose. Repeatability relative standard deviation (RSDs) between 16% at low and 4% at higher contaminations were obtained. The reproducibility RSDs were mostly between 12% and 32%. The trueness of results for T-2 toxin and Zearalenone were not different from 100%, for Deoxynivalenol and HT-2 toxin they were larger than 89%. The extraction was also adapted to a quick screening of Aflatoxin B1 in maize by flow-injection-mass spectrometry. Semi-quantitative results were obtained through standard addition and scan-based ion ratio calculations. The method proved to be a viable greener and quicker alternative to existing methods.
外文关键词：mycotoxins；LC-MS；green analytical chemistry
作者：Breidbach, A
作者单位：European Commiss
期刊名称：TOXINS
期刊影响因子：3.029
出版年份：2017
出版刊次：3
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