中文摘要：本文研究了两个系列的香豆素氨基硫脲对黄曲霉NRRL 3251的抗真菌和抗产黄曲霉素的效果。结果显示，黄曲霉暴露于选定化合物的氧化应激反应表明氧化应激调制可能是抑制黄曲霉毒素生产的一个原因。
外文摘要：The antifungal and antiaflatoxigenic effects of two series of coumarinyl thiosemicarbazides on Aspergillus flavus NRRL 3251 were studied. Fungi were grown in YES medium for 72 h at 29 degrees C in the presence of 0, 0.1, 1, and 10 mu g mL(-1) of coumarinyl thiosemicarbazides: one series with substitution in position 7 and another with substitution in position 4 of the coumarin core. Dry mycelia weight determination was used for antifungal activity estimation, while the aflatoxin B1 content in YES media, determined by the dilute and shoot LC-MS/MS technique, was used for the antiaflatoxigenic effect estimation. Standard biochemical assays were used for oxidative status marker (TBARS, SOD, CAT, and GPX) determination in A. flavus NRRL 3251 mycelia. Results show that 7-substituted-coumarinyl thiosemicarbazides possess a better antifungal and antiaflatoxigenic activity than 4-substituted ones. The most prominent substituted compound was the compound 3,N-(4-chlorophenyl)-2-(2-((4-methyl-2-oxo-2H-chromen-7-yl) oxy) acetyl) hydrazine-1-carbothioamide, which completely inhibited aflatoxin production at the concentration of 10 mu g mL(-1). Oxidative stress response of A. flavus exposed to the selected compounds points to the modulation of oxidative stress as a possible reason of aflatoxin production inhibition.
外文关键词：coumarins；aflatoxins；antimycotoxigenic activity；oxidative status modulation；dilute and shoot LC-MS/MS technique
作者：Kovac, Tihomir；Kovac, Marija；Strelec, Ivica
作者单位：University of JJ Strossmayer Osijek
期刊名称：ARHIV ZA HIGIJENU RADA I TOKSIKOLOGIJU-ARCHIVES OF INDUSTRIAL HYGIENE AND TOXICOLOGY
期刊影响因子：0.971
出版年份：2017
出版刊次：1
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