中文摘要：禾谷镰刀菌是北欧和亚洲最重要的脱氧雪腐镰刀菌烯醇(DON)产生菌。在芬兰发现燕麦粒中的DON含量最高，而禾谷镰刀菌DNA含量与用可靠的GC-MS法测得的DON含量一致。除DON外，芬兰粮食样品中还存在3ADON。当使用RIDA®QUICK扫描试剂盒测得的DON含量的结果时，检测到燕麦中DON含量及禾谷镰刀菌DNA量的变化很大且二者间的测定系数(R2)较差 。本研究印证了当燕麦粉不是用0.8mm或1mm筛研磨时，从中提取的DNA或DON，测定系数通常都较低。芬兰燕麦、大麦及小麦样品中，用Rida®Quick 法测得的DON水平通常高于可靠的GC-MS的结果。燕麦粉通过筛分的均匀化很可能与DON检测中的变化相关联。接近法规限制的禾谷镰刀菌DNA与DON的量应该再次用可靠的定量分析进行证实。
外文摘要：Fusarium graminearum is the most important deoxynivalenol (DON) producing species in northern Europe and Asia. The highest DON levels in Finland have been found in oat grain, and DNA levels of F. graminearum are in agreement with DON levels, when DON is measured by accredited GC-MS. In addition to DON, 3ADON is present in Finnish grain samples. Large variations in DON content and amounts of F. graminearum DNA, and poor coefficient of determination (R2) between these, have been detected in oat grain when the RIDA®QUICK SCAN kit results for DON content have been used. This study confirmed that the coefficient of determination was usually less when DNA or DON were extracted from oat flour, which was not ground with 0.8 mm or 1 mm sieves. DON levels obtained with the Rida®Quick method were usually higher than those obtained with accredited GC-MS in Finnish oat, barley and wheat samples. The homogenization of the oat flour by sieving is therefore likely to be connected to the variations in DON detection. Amounts of F. graminearum DNA and DON close to legislative limits should be reconfirmed with accredited quantitative analyses.
外文关键词：oat；barley；wheat；mycotoxins；qPCR
作者：Yli-Mattila, T；Ramo, S；Hussien, T；Rauvola, M；Hietaniemi, V；Kaitaranta, J
作者单位：Univ Turku
期刊名称：PHYTOPATHOLOGIA MEDITERRANEA
期刊影响因子：1.042
出版年份：2017
出版刊次：1
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