中文摘要：伏马菌素B1（FB1）是由多种镰刀菌产生的霉菌毒素，还是一种全球常见的玉米制品污染物。筛选并利用降解FB1的微生物有望成为解决其对人畜毒性问题的方法。以玉米自然发酵为基础的高水分玉米青贮，可能是这种微生物有意思的储存库。本研究体外模拟FB1自然污染的玉米青贮模型，证明了在青贮过程中，原料中的FB１因生物降解而显著减少。FB１污染的高水分玉米粒的发酵导致毒性明显降低并分离出降解FB１的微生物，进一步用作FB１净化剂。
外文摘要：BACKGROUND: Fumonisin B-1 (FB1) is a mycotoxin produced by several Fusarium species and is a very common contaminant of maize-based food and feed throughout the world. The selection and use of FB1-degrading microorganisms appears as a promising alternative to cope with the problem of toxicity towards humans and livestock. High moisture maize grain silage, which is based on natural maize fermentation, could be an interesting reservoir of such microorganisms.
RESULTS: Using an in vitro simulated silage model with FB1 naturally contaminated grains, we demonstrated a significant raw decrease in FB1 during ensiling process ascribed to biodegradation mechanisms. A panel of 98 bacteria and yeasts were isolated from this matrix and selected for their ability to use FB1 as the sole source of C and N. For nine of them, the ability to degrade FB1 in vitro was evidenced. Notably, two bacteria identified as Lactobacillus sp. were highlighted for their efficient FB1-degrading capacity and production of hydrolysed FB1 as intermediate degradation metabolite.
CONCLUSION: Fermentation of high moisture maize grain contaminated with FB1 leads to a significant reduction of the toxin and allows the isolation of FB1-degrading microorganisms that could further be used as FB1 decontaminating agents.
外文关键词：biodegradation；mycotoxin；fumonisin B-1；hydrolysed FB1；silage
作者：Tuppia, CM；Atanasova-Penichon, V；Chereau, S；Ferrer, N；Marchegay, G；Savoie, JM；Richard-Forget, F
作者单位：INRA
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
期刊影响因子：2.076
出版年份：2017
出版刊次：8
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