中文摘要：我们最近设计了用于检测产黄曲霉毒素菌株的灵敏且简单可视的方法—敌敌畏-氨（DV-AM）法。该方法能够通过阳性菌落背面的亮紫红色显著区分红色（阳性，产黄曲霉毒素）菌落和淡黄色（阴性，不产黄曲霉毒素）菌落。研究表明，DV-AM方法对直接分离野外样品中产黄曲霉毒素和不产黄曲霉毒素真菌是实际可用的。
外文摘要：We recently devised the dichlorvos–ammonia (DV–AM) method, a sensitive and simple visual method for detecting aflatoxigenic strains. The DV–AM method enables a prominent distinction of red (positive; aflatoxin-producing) colony from pale-yellow (negative; non-aflatoxigenic) colony via brilliant purple-red color of the backside of positive colonies. We used the DV–AM method to screen aflatoxigenic fungi from soil samples in Tsukuba, Ibaraki, Japan. We herein isolated seven positive (red) colonies and four negative (pale-yellow) colonies from one of the 100 independent samples. Chemical analyses demonstrated that these red isolates produced aflatoxins B1 and B2 without aflatoxins G1 nor G2. In contrast, 4 pale-yellow colonies with similar phenotypes to the Aspergillus flavus group were confirmed to be non-aflatoxigenic fungi. Morphological and phylogenic analyses of one positive strain (HA9-S1-1) indicated that it was A. flavus or its closely related species of Aspergillus. These aflatoxigenic strains showed differences in aflatoxin production, morphological characteristics, and sclerotia formation, suggesting that different types of aflatoxigenic strains were present in the same soil. These results demonstrated that the DV–AM method is practically useful for the direct isolation of aflatoxigenic and non-aflatoxigenic fungi from field samples.
外文关键词：Aflatoxin；Ammonia vapor；Aspergillus flavus；Section Flavi
作者：Kushiro, M；Hatabayashi, H；Zheng, YZ；Yabe, K
作者单位：Natl Agr & Food Res Org NARO
期刊名称：MYCOSCIENCE
期刊影响因子：1.165
出版年份：2017
出版刊次：2
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