中文摘要：本研究旨在评估百里香（Thymus vulgaris）精油对尖孢镰刀菌（F. oxysporum）菌株生长和Tri4基因表达的作用。该精油用Clevenger型系统通过水蒸馏法获得。通过气相色谱-质谱法（GC-MS）和保留指数法获得精油的化学成分。通过肉汤微量稀释法评估抗真菌活性。基于单端孢生物合成基因Tri4，研发了对尖孢镰刀菌特异性的定量实时RT-PCR（qRT-PCR）测定法，其允许从尖孢镰刀菌中进行鉴别。结果表明，可以将百里香精油视为替代目前预防和控制种传病害的合成化学品的天然杀真菌剂。
外文摘要：The aims of this study were to evaluate the efficacy of Thymus vulgaris (T. vulgaris) essential oil on the fungal growth and Tri4 gene expression in Fusarium oxysporum (F. oxysporum) strains. The oil was obtained by water-distillation using a Clevenger-type system. The chemical composition of the essential oil was obtained by gas chromatography- mass spectroscopy (GC-MS) and by retention indices. The anti fungal activity was evaluated by broth microdilution assay. A quantitative real time RT-PCR (qRT-PCR) assay was also developed specific for F oxysporum on the basis of trichothecene biosynthetic gene, Tri4, which allowed discrimination from F. oxysporum. Results showed thymol (32.67%) and p-cymene (16.68%) as the main components of T vulgaris. Minimum inhibitory concentration (MIC) values varied from 5 to 20 mu g/ml with T vulgaris (mean: 10.50 mu g/ml), while minimum fungicidal concentration (MFC) values ranged from 8 to 30 mu g/ml with mean value of 16.20 mu g/ml qRT-PCR results revealed a down regulation from 4.04 to 6.27 fold of Tri4 gene expression of the fungi exposed to T. vulgaris essential oil. The results suggest that T. vulgaris oil can be considered potential alternative natural fungicide to the synthetic chemicali that are currently used to prevent and control seed-borne diseases.
外文关键词：Fusarium oxysporum；Thymus vulgaris；Essential oil；Real time PCR；Tri4 gene
作者：Divband, K；Shokri, H；Khosravi, AR
作者单位：Univ Tehran
期刊名称：MICROBIAL PATHOGENESIS
期刊影响因子：1.888
出版年份：2017
出版刊次：3
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