中文摘要：本研究开展了两个试验：优化纤维素微球菌（C.funkei）对棉籽粕的发酵，提高细菌降解黄曲霉毒素B1（AFB1）的能力；评估小鸭的细菌解毒作用。结果表明用C.funkei发酵受AFB1污染的饲料原料，为减轻幼鸭因黄曲霉毒素中毒受到的负面影响提供了一种新的措施。
外文摘要： Two experiments were conducted to optimize the fermentation of cottonseed meal by Cellulosimicrobium funkei (C. funkei) for the ability of the bacteria to degrade aflatoxin B1 (AFB1) and then to evaluate the bacterial detoxification in ducklings. In experiment 1, the fermentation of cottonseed meal by C. funkei was improved by changing the inoculation amounts by 10% (10(8) cfu/mL), using a 1: 0.5 material to water ratio at 35 degrees C temperature for a 144 h reaction duration, which resulted in an 83.4% biodegradation of AFB1. In experiment 2, 112 one-day-old male Cherry Valley ducklings were randomly allocated to 4 experimental groups with 4 replicates of 7 birds each. For a period of 2 wk the controls received a base duckling diet (BD), a second group received a base diet contaminated with 10% AFB1 cottonseed meal (96.8 mu g AFB1/kg), a third group was fed a base diet added with 5% unfermented and 5% fermented AFB1-contaminated cottonseed meal (57.0 mu g AFB1/kg), and the fourth group was fed a base diet added with 10% AFB1-contaminated fermented cottonseed meal (16.0 mu g AFB1/kg). The growth performance, relative organ weights, and serum biochemistry were analyzed. The results showed that the feed conversion ratio in the second group was lower than that of the controls at wk one and 2 (P < 0.05). Also, after 2 wk, group 2 ducklings had increased relative weights of the liver, kidneys, and spleen, increased activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), increased concentration of blood urea nitrogen (BUN) and creatinine (Crt), and decreased relative weight of Fabricius bursa (P < 0.05). In addition, the concentrations of total protein (TP) and albumin (ALB) in serum were also significantly higher at weeks one and 2 (P < 0.05). These alterations were attenuated or prevented when 5 or 10% fermented cottonseed meal substituted equal amounts of unfermented cottonseed meal in the diet. In conclusion, fermentation of AFB1-contaminated feed materials by C. funkei offers a new strategy to reduce the negative effects of aflatoxicosis in ducklings.
外文关键词：aflatoxin B1；Cellulosimicrobium funkei；fermentation；cottonseed meal；duckling
作者：Liu, J；Song, WJ；Zhang, NY；Tan, J；Krumm, CS；Sun, LH；Qi, DS
作者单位：Huazhong Agr Univ
期刊名称：POULTRY SCIENCE
期刊影响因子：1.685
出版年份：2017
出版刊次：4
点击下载：通过纤维素微球菌发酵鸭饲料，对棉籽粕中的黄曲霉毒素B1进行生物脱毒