中文摘要：黄曲霉毒素B1（AFB1）是一种由曲霉属真菌，特别是黄曲霉产生的致癌物质。几毫微克的AFB1，通过皮肤渗透后足以引发肝癌和生长发育迟缓。在本研究利用氧化石墨烯（GO）的荧光淬灭特性和一种荧光素amidite（FAM）修饰的对AFB1具有特异性的适体，开发了一种AFB1的快速适体检测法。研究发现，由于该生物传感器检测范围广的且测定迅速，认为它与常规检测方法一样可用于现场检测。
外文摘要：Aflatoxin B-1 (AFB(1)) is a carcinogenic substance produced by fungi of genus Aspergillus, especially Aspergillus flavus. Few nanograms of AFB(1) that permeated through the skin is sufficient to cause liver cancer and stunted growth. In this study, a rapid aptamer-based assay for AFB(1) was developed using the fluorescence quenching property of graphene oxide (GO) and a fluorescein amidite (FAM)-modified aptamer specific to AFB(1). The aptamer, modified with the fluorescence dye FAM on its 5'-end, was used as a probe. Once bound by AFB(1), a conformational change of the aptamer was caused that led to its interaction with the well-known fluorescence quencher GO, resulting in a decrease of the fluorescence intensity of the system.In the absence of AFB(1), the fluorescence intensity remained unchanged. The aptamer-based AFB(1) assay process was conducted through 3 steps within 40 min. The aptamer was incubated with AFB(1) before the addition of GO. The amount of AFB(1) present was measured by the change in fluorescence intensity. The detection system was evaluated with standard solutions of AFB(1) of various concentrations. The results showed that the fluorescence intensity decreased linearly as the concentration of AFB(1) gradually increased. Although the assay was specific to AFB(1), there was slight interference by other types of aflatoxin. When the assay was applied to a real sample, the limit of detection was 4.5 ppb, which was within the wide detection range of up to 300 ppb with good linearity. Thus, this biosensor is considered to be competitive with the conventional detection methods in the field owing to its wide detection range and assay rapidity.
外文关键词：Aflatoxin B1；Molecular detection；Fluorescence quenching；Graphene oxide
作者：Joo, M；Baek, SH；Cheon, SA；Chun, HS；Choi, SW；Park, TJ
作者单位：Chung Ang Univ
期刊名称：COLLOIDS AND SURFACES B-BIOINTERFACES
期刊影响因子：3.902
出版年份：2017
出版刊次：27
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