中文摘要：本研究确定了氯甲酸-9-芴基甲酯(FMOC-Cl)柱前衍生能有效测定饲料中的伏马菌素B1和B2。液固萃取、免疫亲和固相萃取色谱法净化、FMOC衍生后进行反相HPLC荧光分析。研究表明FMOC衍生是一种适用于量化谷物饲料中伏马菌素B1和B2的方法。
外文摘要：Pre-column derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) was determined to be effective for quantitation of fumonisins B1 and B2 in feed. Liquid-solid extraction, clean-up using immunoaffinity solid phase extraction chromatography, and FMOC-derivatization preceded analysis by reverse phase HPLC with fluorescence. Instrument response was unchanged in the presence of matrix, indicating no need to use matrix-matched calibrants. Furthermore, high method recoveries indicated calibrants do not need to undergo clean-up to account for analyte loss. Established method features include linear instrument response from 0.04–2.5 µg/mL and stable derivatized calibrants over 7 days. Fortified cornmeal method recoveries from 0.1–30.0 μg/g were determined for FB1 (75.1%–109%) and FB2 (96.0%–115.2%). Inter-assay precision ranged from 1.0%–16.7%. Method accuracy was further confirmed using certified reference material. Inter-laboratory comparison with naturally-contaminated field corn demonstrated equivalent results with conventional derivatization. These results indicate FMOC derivatization is a suitable alternative for fumonisins B1 and B2 quantitation in corn-based feeds.
外文关键词：Fumonisin B-1；Fumonisin B-2；FMOC derivatization；Mycotoxins
作者：Smith, LL；Francis, KA；Johnson, JT；Gaskill, CL
作者单位：Univ Kentucky
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：11
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