中文摘要：霉菌毒素是真菌产生的次生代谢物。它们通常多重污染食品和饲料商品，构成人畜健康风险。认为快捷简便的多重筛选是有用的检测受多重污染的食品和饲料的工具。本研究用UV诱导的共聚合制作了一种高灵敏的免疫亲和单片阵列，用于检测霉菌毒素玉米赤霉烯酮、脱氧雪腐镰刀菌烯醇、T-2毒素、HT-2毒素、黄曲霉毒素、赭曲霉素A及伏马菌素。结果证明了在实际食品样品检测中，该霉菌毒素免疫亲和单片阵列是一种灵敏、稳定且经济的工具。此外，免疫亲和单片阵列能简化操作步骤：加样和酶标霉菌毒素，并检测CL信号。
外文摘要：BACKGROUND Mycotoxins are produced by fungi as secondary metabolites. They often multi-contaminate food and feed commodities posing a health risk to humans and animals. Fast and easy multiplex screening could be thought as a useful tool for detection of multi-contaminated food and feed commodities. RESULTS A highly sensitive immune-affinity monolithic arrays for detecting the mycotoxins zearalenone, deoxynivalenol, T-2 toxin, HT-2 toxin, aflatoxins, ochratoxin A, and fumonisin B1 were fabricated using UV induced co-polymerisation. The mycotoxin antibodies firstly reacted with functional monomer to form antibody/functional monomer bio-conjugates. Subsequently, the antibody/functional monomer bio-conjugates co-polymerised with cross-linker to form mycotoxins immune-affinity arrays. With optimal fabrication conditions, all mycotoxin immune-affinity monolithic arrays exhibited a linear response spanning three orders of magnitude. And the immune-affinity monolithic array has a low detection limit and has a good uniformity (intra-assay CV, and inter-assay CV both CONCLUSION The fabricated mycotoxin immune-affinity monolithic arrays were proved as a sensitive, stable and economical tool in real food samples detection. Moreover, the mycotoxin immune-affinity monolithic arrays would be able to minimise manipulation steps: add samples and enzyme labelled mycotoxins, and detect CL signals.
外文关键词：immune-affinity array；mycotoxins；chemiluminescent detection；monolith；co-polymerisation
作者：Li, L；Xia, LR；Zhao, YF；Wang, M；Jiang, XZ
作者单位：Jiangsu Acad Agr Sci
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
期刊影响因子：2.076
出版年份：2017
出版刊次：8
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