中文摘要：本研究首次提出了一种基于无镉量子点（QDs）的同步定性检测两种霉菌毒素的灵敏工具。已经证明了QDs是有前景的用于生物标记和化学分析的荧光标记。随着全球规定和限制使用有害元素的趋势日益增强，基于Pb或者Cd的QDs虽然高效并已得到充分研究，但具有潜在毒性，就凸显出磷化铟（InP）QDs是一种环保的替代品。本研究便开发了基于水溶性InP QDs的荧光纳米材料。
外文摘要：A sensitive tool for simultaneous qualitative detection of two mycotoxins based on use of non-cadmium quantum dots (QDs) is presented for the first time. QDs have proven themselves as promising fluorescent labels for biolabeling and chemical analysis. With an increasing global tendency to regulate and limit the use of hazardous elements, indium phosphide (InP) QDs are highlighted as environmentally-friendly alternatives to the highly efficient and well-studied, but potentially toxic Cd-and Pb-based QDs. Here, we developed water-soluble InP QDs-based fluorescent nanostructures. They consisted of core/shell InP/ZnS QDs enrobed in a silica shell that allowed the water solubility (QD@SiO2). Then we applied the QD@SiO2 as novel, silica shell -encapsulated fluorescent labels in immunoassays for rapid multiplexed screening. Two mycotoxins, zearalenone and deoxynivalenol, were simultaneously detected in maize and wheat, since the two QD@SiO2 labelled conjugates emit at two different, individually detectable wavelengths. The cutoff values for the simultaneous determination were 50 and 500 mu g kg(-1) for zearalenone and deoxynivalenol, respectively, in both maize and wheat. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used to confirm the result.
外文关键词：Quantum dots；Indium phosphide (InP)；Cadmium-free；Lateral flow immunoassay；QD@SiO2；Mycotoxins
作者：Beloglazova, Natalia V.；Sobolev, Aleksander M.；Tessier, Mickael D.等
作者单位：Ghent University
期刊名称：METHODS
期刊影响因子：3.503
出版年份：2017
出版刊次：-
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