中文摘要：玉米易受黄曲霉感染，而后者产生黄曲霉毒素污染产品。本研究旨在分析水活度(aw)与温度间的相互作用对黄曲霉菌株在玉米培养基中的生长、生物合成调节基因(aflR)的表达及产生黄曲霉毒素B1(AFB1)的影响。本研究提供的资料有助于更好地了解高风险AFB1出现的条件。但是，单靠aflR的表达来指示AFB1的产生还不够。因此，应进一步进行其它AF相关基因的分子研究。这些结果在玉米的收获和储存以及当时尽量减少AFB1污染的环境条件的上下文中进行了讨论。
外文摘要：Maize is prone to infection by Aspergillus flavus, which can contaminate the product with aflatoxins. The objective of this study was to examine the impact that interactions between water activity (aw) and temperature may have on growth, the expression of a biosynthetic regulatory gene (aflR) and aflatoxin B1 (AFB1) production by a strain of A. flavus on a maize-based medium. Results showed that there were some differences between lag phases and growth rates of A. flavus. The optimum growth rate for A. flavus was at 30 °C and 0.99 aw. No growth occurred at 0.90 aw and 20 °C. Both temperature and aw had an influence on the relative aflR gene expression and AFB1 production by A. flavus; however, the results for AFB1 production were not consistent with the effects on gene expression or growth. These findings provide data that are useful to better understand the conditions which represent higher risks from AFB1 production. However, the aflR expression was not a good indicator of AFB1 production alone. Thus, further molecular studies of other AF-related genes should be done. These results are discussed in the context of harvesting and storage of maize and the prevailing environmental conditions to minimise AFB1 contamination.
外文关键词：Maize；Aflatoxin；Aspergillus flavus；aflR, Environment
作者：Bernaldez, V；Cordoba, JJ；Magan, N；Peromingo, B；Rodriguez, A
作者单位：Univ Extremadura
期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY
期刊影响因子：2.711
出版年份：2017
出版刊次：9
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