中文摘要：黄曲霉与寄生曲霉是谷物，特别是玉米及其它农产品中最常见的与黄曲霉毒素(AF)污染相关的真菌。AFB1，最常见、毒性最强的代谢物，有很强的肝毒性，能致畸、致突变。亟需有效控制食品及饲料中这些真菌及黄曲霉毒素的措施。含精油(EO)的活性包装膜是一种最具创新性的食品包装理念。本研究研发了分别结合了牛至(ORE)、肉桂(CIN)的EO或它们的主要活性成分香荆芥酚(CAR)和肉桂醛(CINHO)的乙烯-乙烯醇共聚物(EVOH)薄膜，并测试其在不同aw及温度情况下对玉米粒中黄曲霉及寄生曲霉生长以及AF产生的控制。这是首次研究环境条件之间相互作用及生物活性EVOH-CINHO、EVOH-ORE、EVOH-CIN EVOH-CAR膜对玉米粒中产黄曲霉毒素真菌及AF产生的影响。
外文摘要：Aspergillus flavus and A. parasiticus are the most common fungal species associated with aflatoxin (AF) contamination of cereals, especially maize, and other agricultural commodities. AFB1, the most frequent and toxic metabolite, is a powerful hepatotoxic, teratogenic and mutagenic compound. Effective strategies to control these fungal species and AFs in food and feed are required. Active packaging film containing essential oils (EO) is one of the most innovative food packaging concepts. In this study, ethylene-vinyl alcohol (EVOH) copolymer films incorporating EO from Origanum vulgare (ORE), Cinnamomum zeylanicum (CIN) or their major active constituents, carvacrol (CAR) and cinnamaldehyde (CINHO), respectively, were developed and assayed to control growth of A. flavus and A. parasiticus and AF production in maize grains under different aw and temperature regimens. EO doses assayed in cultures were in the range 0.25-4.0mg/Petri dish. The factors aw, temperature, type of EVOH-EO film and fungal species significantly influenced the ED50 values of all assayed films. Growth rate (GR) of both species was usually higher at 0.99 than at 0.96 aw and at 37°C than at 25°C. However, the contrary was found with regard to AF production. The order of efficacy of EVOH-EO films to control growth of both species and AF production was EVOH-CINHO>EVOH-CAR>EVOH-ORE>EVOH-CIN. The effective dose (ED50) (mg EO/plate) for EVOH-CINHO and EVOH-CIN films against A. flavus were in the ranges of 0.125 and 2.475-3.500 and against A. parasiticus in the ranges of 0.121-0.133 and 2.275-3.625, respectively. Under the assayed conditions, the ED90 for EVOH-CINHO film were 0.22-0.23mg/plate for both species. It was the most effective bioactive film to control fungal growth (vapour phase) and AF production, regardless of aw and temperature. This is the first study about the impact that interacting environmental conditions and bioactive EVOH-CINHO, EVOH-ORE, EVOH-CIN EVOH-CAR films have on the growth of aflatoxigenic fungi and on AF production in maize grains.
外文关键词：Bioactive ethylene-vinyl alcohol copolymer (EVOH)； Aspergillus flavus；Aspergillus parasiticus；Aflatoxins；Essential oils；Maize
作者：Mateo, EM；Gomez, JV；Dominguez, I；Gimeno-Adelantado, JV；Mateo-Castro, R；Gavara, R；Jimenez, M
作者单位：Univ Valencia
期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
期刊影响因子：3.445
出版年份：2017
出版刊次：8
点击下载：基于EVOH薄膜和精油的生物活性包装系统对控制玉米中产黄曲霉毒素真菌及黄曲霉毒素产生的影响