中文摘要：真菌毒素和抗菌剂是导致多种健康问题的主要有害化学物质。现在，多重免疫测定是全球的主要目标。本研究选择黄曲霉毒素M1和吡利霉素作为模型，开发了一种新型双重比色编码的frit免疫测定法，用于同时筛选牛奶中的黄曲霉毒素M1和吡利霉素残留物。研究表明，该新型比色frit免疫测定法具有高通量、分析时间短、降低每次检测的总成本的优点，可用作同时检测牛奶中黄曲霉毒素M1和吡利霉素残留的快速筛选技术。
外文摘要：Mycotoxins and antibacterial agents are the main chemical hazards that lead to several health problems. Nowadays, multiplex immunoassay is a primary goal throughout the world. Here, aflatoxin M-1, and pirlimycin were selected as models, and a novel dual colorimetric encoded frit-based immunoassay was developed for simultaneously screening of aflatoxin M-1, and pirlimycin residues in milk. This multiplex fit-based immunoassay combined two monoclonal antibodies to extend the spectrum of analytes and to enable detection of two classes of analytes in a single test. The cutoff values were 0.02 mu g/kg for aflatoxin M-1, and 0.5 mu g/kg for pirlimycin, which satisfied the requirement to measure the maximum residue levels. The novel colorimetric frit-based immunoassay has the advantage of high throughput, short analysis time, reduced overall cost per assay, and can be used as a rapid screening technique for simultaneously detecting aflatoxin M-1 and pirlimycin residues in milk.
外文关键词：aflatoxin M-1；pirlimycin；immunoassay；milk；food safety
作者：Jiang, WX；Beloglazova, NV；Luo, PJ；Guo, P；Lin, GM；Wang, XM
作者单位：Shenzhen Univ
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
期刊影响因子：2.857
出版年份：2017
出版刊次：8
点击下载：用于视觉检测牛奶中黄曲霉毒素M1和吡利霉素残留的双色量子点编码的frit免疫测定法