中文摘要：本文研究了从突尼斯收集的65个储存和新收的小麦、大麦和玉米样品中，黄曲霉毒素（AFs）AFB1、AFB2、AFG1、AFG2和赭曲霉毒素A（OTA）的发生情况。用高效液相色谱法同时提取并定量分析真菌毒素。通过种特异性聚合酶链反应（PCR）测定这些样品中产AF（Flavi组）和产OTA的曲霉菌（Nigri组和环绕亚属）。结果表明，大多数玉米样品都受到AFs的污染，贮藏后的含量比收获时低。所有被污染的玉米样品均含有AFG1和AFG2，其中27.78％含有AFB1和AFB2。这个AFs模式与寄生黄曲霉毒素的分布一致。而黄曲霉在玉米中的出现频率最高，在大麦和小麦中也发现黄曲霉但没有检测到AFs。相反，在玉米和大麦中都没有检测到OTA，只有一个小麦样品含有OTA。检测到黑曲霉是唯一产生OTA的种类。
外文摘要：Occurrence of aflatoxins (AFs) AFB1, AFB2, AFG1, AFG2 and ochra toxin A (OTA) was studied in 65 samples of stored and freshly harvested wheat, barley and maize collected in Tunisia. The mycotoxins were simultaneously extracted and quantified by high performance liquid chromatography. Determination of AF-producing (section Flavi) and OTA-producing Aspergillus species (sections Nigri and Circumdati) was conducted in these samples by species-specific polymerase chain reaction (PCR). Results showed that most of maize samples were contaminated with AFs, data after storage showing lower values than those collected at harvest. All contaminated maize samples contained AFG1 and AFG2, among which 27.78% also had AFB1 and AFB2. This AFs pattern was consistent with the A. parasiticus toxin profile. A. flavus however showed the highest frequency in maize but was also found in barley and wheat where no AFs were detected. In contrast, OTA was neither found in maize nor in barley and only one wheat sample contained OTA. A. niger was the only OTA-producing species detected.
外文关键词：Wheat；barley；maize；aflatoxin B1；aflatoxin B2；aflatoxin G1；aflatoxin G2；ochratoxin A；toxigenic Aspergillus species；PCR
作者：Jedidi, I；Cruz, A；Gonzalez-Jaen, MT；Said, S
作者单位：Univ Sousse
期刊名称：FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE
期刊影响因子：1.467
出版年份：2017
出版刊次：1
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