中文摘要：T-2毒素造成的危害已经引起越来越多的关注，因此精确分析包括虾在内的水生生物总T-2毒素（游离及隐蔽型）很重要。所以精确分析虾组织中游离T-2特别是隐蔽型T-2很重要。本研究用免疫磁珠酶联免疫吸附测定(IMB-ELISA)，开发了一种快捷、灵敏且有效的定量检测游离T-2及隐蔽型T-2的方法。研究表明ELISA结合免疫磁珠技术提供了一种新的、方便的方法，显著改善了虾组织中总T-2的检测精度及灵敏度。
外文摘要：Accurate analyses of total T-2 (free and modified) in aquatic organisms including shrimp are important as the hazard caused by T-2 has been caught increasing attention. Therefore, acurate analysis of free T-2 especially of modified T-2 in shrimp tissues is important. A rapid, sensitive, and validated method for quantitative determination of free T-2 and modified T-2 toxin was developed using immunomagnetic-bead based enzyme-linked immunosorbent assay (IMB-ELISA). Super paramagnetic particles with a carboxyl group activated by an ester method coupled with envelope antigen 3- acetylneosolaniol- hemisuccinate - ovalbumin (3-Ac-NEOS-HS-OVA) was used to form immunomagnetic beads which could bind to T-2 skeletal structure antibodies. The conditions for magnetic bead coating of T-2 skeletal structure antibodies, and the concentrations of the polyclonal antibody and HRP-labeled goat anti-rabbit antibody were optimized. A good linear relationship with T-2 concentrations ranging from 5-75ng/mL (R2 =0.9965) was observed. The detection limit of different shrimp tissues of the IMB-ELISA ranged from 2.53 to 3.20ng/mL. And the IC50 was 63ng/mL. The recovery varied from 86% to 99% with a standard deviation of 2.8-5.8%. The application of this method to study the distribution in tissues showed that the total T-2 concentration in hepatopancreas was 26.7µg/kg > blood > head > muscle in the highest dose group of 12.2mg/kg. Our research showed a combination of ELISA and immunomagnetic bead technology provide a new, convenient approach to significantly improve the accuracy and sensitivity of total T-2 measurement in shrimp tissues.
外文关键词：T-2 toxins；Immunomagnetic；ELISA；Shrimp tissues
作者：Deng, Q；Qiu, M；Wang, YL；Lv, PL；Wu, CJ；Sun, LJ；Ye, RY；Xu, DF；Liu, Y；Gooneratne, R
作者单位：Guangdong Ocean Univ
期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
期刊影响因子：3.13
出版年份：2017
出版刊次：8
点击下载：一种灵敏而有效的分析虾组织中总T-2毒素（游离及隐蔽型）的免疫磁珠酶联免疫吸附测定法