中文摘要:层生镰刀菌不仅在玉米中,还在包括小麦的各种作物中产生伏马菌素B1。用小麦培养基,比较了非生物因素、温度及水活性(aW)对三株阿根廷硬质小麦层生镰刀菌的生长、伏马菌素生物合成及FUM基因表达的影响。本研究提供了有用的代表着小麦粒受伏马菌素污染风险高或低的条件的基线数据。
外文摘要:Fusarium proliferatum produces fumonisins B not only on maize but also on diverse crops including wheat. Using a wheat-based medium, the effects of abiotic factors, temperature and water activity (aW), on growth, fumonisin biosynthesis, and expression of FUM genes were compared for three F. proliferatum strains isolated from durum wheat in Argentina. Although all isolates showed similar profiles of growth, the fumonisin production profiles were slightly different. Regarding FUM gene transcriptional control, both FUM8 and FUM19 expression showed similar behavior in all tested conditions. For both genes, expression at 25 °C correlated with fumonisin production, regardless of the aw conditions. However, at 15 °C, these two genes were as highly expressed as at 25 °C although the amounts of toxin were very weak, suggesting that the kinetics of fumonisin production was slowed at 15 °C. This study provides useful baseline data on conditions representing a low or a high risk for contamination of wheat kernels with fumonisins.
外文关键词:Fumonisin;Fusarium proliferatum;Wheat;FUM gene;Gene expression;Abiotic stress
作者:Cendoya, E;Pinson-Gadais, L;Farnochi, MC;Ramirez, ML;Chereau, S;Marcheguay, G;Ducos, C;Barreau, C;Richard-Forget, F
作者单位:Univ Nacl Rio Cuarto
期刊名称:INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
期刊影响因子:3.445
出版年份:2017
出版刊次:7
点击下载:引起小麦基质层生镰刀菌菌株的FUM基因表达及伏马菌素积累的非生物环境