中文摘要：本研究验证并应用了一种简便快捷的方法分析不同的乳制品中一组由22种雌激素组成的混合物，包括八种植物雌激素（即大豆甙元、肠二醇、大豆黄素、肠内酯、染料木黄酮、芒柄花素、樱黄素、鹰嘴豆芽素A），六种霉菌毒素（β-玉米赤霉醇、β-玉米赤霉烯醇、α-玉米赤霉醇、α-玉米赤霉烯醇、玉米赤霉酮、玉米赤霉菌烯酮）以及四种合成物（即炔雌醇、己烯雌酚、双烯雌酚、己烷雌酚）和四种天然雌激素（即雌三醇、17β-雌二醇、17α-雌二醇、雌酮）。用该方法分析市售产品，发现某些样品中存在大豆甙元、大豆黄素、肠内酯及染料木黄酮。
外文摘要：In this work, a simple and fast methodology has been validated and applied for the analysis of a group of 22 estrogenic compounds including eight phytoestrogens (i.e. daidzein, enterodiol, glycitein, enterolactone, genistein, formononetin, prunetin, biochanin A), six mycotoxins (β-zearalanol, β-zearalenol, α-zearalanol, α-zearalenol, zearalanone, zearalenone) as well as four synthetic (i.e. ethynylestradiol, diethylstilbestrol, dienestrol, hexestrol) and four natural estrogens (i.e. estriol, 17β-estradiol, 17α-estradiol, estrone) in different dairy products. Extraction was carried out using the QuEChERS method while separation, determination and quantification of the target analytes were achieved by ultra-high-performance liquid chromatography coupled to triple quadrupole mass spectrometry with an electrospray ionization interface. The methodology was validated for four dairy product samples with relevant interest for the population including skimmed and whole cheese and goat and cow kefir, using 17β-estradiol-2,4,16,16,17-d5 as internal standard for natural and synthetic estrogens and β-zeralanol-10,10,11,12,12-d5 as internal standard for mycotoxins and phytoestrogens. Recovery ranged from 70 to 119% for the four types of matrices with RSD values lower than 14% and the limits of quantification of the method achieved were in the range 0.025–2.50 μg/kg for all samples. Finally, the analysis of commercially available products was carried out finding the presence of daidzein, glycitein enterolactone and genistein in some of the studied samples.
外文关键词：Estrogens；QuEChERS；Cheese；Kefir；Ultra-high-performance liquid；chromatography；Mass spectrometry
作者：Socas-Rodriguez, B；Herrera-Herrera, AV；Hernandez-Borges, J；Rodriguez-Delgado, MA
作者单位：ULL
期刊名称：JOURNAL OF CHROMATOGRAPHY A
期刊影响因子：3.926
出版年份：2017
出版刊次：5
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