中文摘要：本研究开发了一种竞争性直接酶联免疫吸附试验(cd-ELISA)用于快速检测食品及饲料中的脱氧雪腐镰刀菌烯醇(DON)。
 
外文摘要：A competitive direct enzyme-linked immunosorbent assay (cd-ELISA) was developed for the rapid detection of deoxynivalenol (DON) in food and feedstuff. Polyclonal antibodies against DON were generated by immunizing rabbits with 3-HS-DON-BSA conjugates. In this assay, the sensitivity (measured as IC50) and limit of detection (measured as IC15) were 0.03 and 0.003 mg kg−1, respectively. A total of eight sample types (barley, wheat, oat, maize, rice, flour, milk and feedstuff) were chosen to evaluate the cd-ELISA assay performance. The sample could be directly detected after extraction and dilution with double-distilled water. The limit of detection in the samples was in the range of 0.15–0.48 mg kg−1. In the end, the spike and recovery results in both cd-ELISA and high-performance liquid chromatography (HPLC) were compared for assay validation. The recovery results ranged between 70% and 100%. A good correlation (R2 = 0.9613) between ELISA and HPLC was obtained.
外文关键词：Deoxynivalenol；competitive direct enzyme-linked immunosorbent assay；foods；feedstuff
作者：Zhang, Y；Yang, J；Lu, Y；Ma, DY；Qi, MG；Wang, S
作者单位：Tianjin Univ Sci & Technol
期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY
期刊影响因子：1.548
出版年份：2017
出版刊次：3
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