中文摘要：伏马菌素（FBs）广泛存在于水稻、玉米、花生、小麦和其它农产品中。这些都是使用色谱技术检测到的，而高度灵敏的单克隆抗体快速测定的报道很少。本文通过杂交瘤技术成功研发出高灵敏度的单克隆抗体（7A11），并在此基础上建立了高灵敏度的间接竞争性酶联免疫吸附测定（icELISA）和基于金纳米粒子的灰度成像定量免疫测定（GNPs-GI）。研究发现，与高效液相色谱法（HPLC）相比，icELISA和GNPs-GI表现出可用于进一步检测农产品中伏马菌素B1的可靠性。
外文摘要：Fumonisins (FBs) are widely found in rice, maize, peanut, wheat, and other agricultural products. These have been detected using a chromatography technique, whereas the rapid assay by a highly sensitive monoclonal antibody is minimally reported. Herein, a highly sensitive monoclonal antibody (7A11) was successfully developed by hybridoma technique. The 50% inhibition concentration (IC50) of 7A11 monoclonal antibody was 0.32 ng/mL in an optimized buffer. The cross-reactivity between fumonisin B1 and fumonisin B2, B3 was 4.3% and 12.8%, respectively. Based on the newly developed 7A11 antibody, a high sensitivity indirect competitive enzyme-linked immunosorbent assay (icELISA) and gold nanoparticles-based gray imaging quantification immunoassay (GNPs-GI) were established. The analytical range of icELISA was 0.08–1.38 ng/mL and that for GNPs-GI was 0.24–15 ng/mL. Both the methods showed adequate recoveries (80.0–105.8% for icELISA and 78.5–115.2% for GNPs-GI) in spiked samples. Compared to high-performance liquid chromatography (HPLC), icELISA and GNPs-GI indicated reliability that could be used for further detection of fumonisin B1 in agricultural products.
外文关键词：Fumonisin B-1； Monoclonal antibody； Gray imaging； Immunoassay； Agricultural product
作者：Tang, XQ； Li, PW； Zhang, ZW； Zhang, Q； Guo, JN； Zhang, W
作者单位：Chinese Acad Agr Sci
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2017
出版刊次：5
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