中文摘要：霉菌毒素会污染包括谷类在内的各种食品。不同类的霉菌毒素可以共同污染食物，增加人体健康风险。已有文献报道了多种测定谷物中霉菌毒素的分析方法。而本研究目的是提出一种简便有效的系统，用于从玉米粉和硬粒小麦粉中提取六种主要的真菌毒素，即主要的四种黄曲霉毒素、赭曲霉毒素A和霉菌类雌激素玉米赤霉烯酮。本方法运用了磁性固相萃取（SPE），这种技术作为传统SPE的替代品，引起了越来越多的兴趣。本文方法结果的定量限与应用于谷物中仅限于一种或两种霉菌毒素的其他两种磁性SPE的方法获得的定量限相当，而本方法研究的霉菌毒素属于三种不同的化学类别。
外文摘要：Mycotoxins can contaminate various food commodities, including cereals. Moreover, mycotoxins of different classes can co-contaminate food, increasing human health risk. Several analytical methods have been published in the literature dealing with mycotoxins determination in cereals. Nevertheless, in the present work, the aim was to propose an easy and effective system for the extraction of six of the main mycotoxins from corn meal and durum wheat flour, i.e., the main four aflatoxins, ochratoxin A, and the mycoestrogen zearalenone. The developed method exploited magnetic solid phase extraction (SPE), a technique that is attracting an increasing interest as an alternative to classical SPE. Therefore, the use of magnetic graphitized carbon black as a suitable extracting material was tested. The same magnetic material proved to be effective in the extraction of mycoestrogens from milk, but has never been applied to complex matrices as cereals. Ultra high-performance liquid chromatography tandem mass spectrometry was used for detection. Recoveries were > 60% in both cereals, even if the matrix effects were not negligible. The limits of quantification of the method results were comparable to those obtained by other two magnetic SPE-based methods applied to cereals, which were limited to one or two mycotoxins, whereas in this work the investigated mycotoxins belonged to three different chemical classes.
外文关键词：mycotoxins； aflatoxins； ochratoxin A； zearalenone； magnetic solid phase extraction； graphitized carbon black； liquid chromatography-tandem mass spectrometry； cereals； wheat； maize
作者：La Barbera, G； Capriotti, AL； Cavaliere, C； Foglia, P； Montone, CM；Chiozzi, RZ； Lagana, A
作者单位：Univ Roma La Sapienza
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：4
点击下载：一种快速磁性固相萃取结合液相色谱-串联质谱分析法用于测定谷物中的霉菌毒素