中文摘要：本文开发了一种具有阳性读数的HT2毒素特异性简单ELISA。该测定法建立在与碱性磷酸酶（AP）基因融合的抗免疫复合物（IC）scFv抗体片段的基础上。研究表明IC ELISA可用于特异性筛选HT2毒素，并用于所测试的全部三种谷物基质的相关浓度范围内。
外文摘要：We developed an HT-2 toxin-specific simple ELISA format with a positive read-out. The assay is based on an anti-immune complex (IC) scFv antibody fragment, which is genetically fused with alkaline phosphatase (AP). The anti-IC antibody specifically recognizes the IC between a primary anti-HT-2 toxin Fab fragment and an HT-2 toxin molecule. In the IC ELISA format, the sample is added together with the scFv-AP antibody to the ELISA plate coated with the primary antibody. After 15 min of incubation and a washing step, the ELISA response is read. A competitive ELISA including only the primary antibody recognizes both HT-2 and T-2 toxins. The anti-IC antibody makes the assay specific for HT-2 toxin, and the IC ELISA is over 10 times more sensitive compared to the competitive assay. Three different naturally contaminated matrices: wheat, barley and oats, were used to evaluate the assay performance with real samples. The corresponding limits of detection were 0.3 ng/mL (13 mu g/kg), 0.1 ng/mL (4 mu g/kg) and 0.3 ng/mL (16 mu g/kg), respectively. The IC ELISA can be used for screening HT-2 toxin specifically and in relevant concentration ranges from all three tested grain matrices.
外文关键词：HT-2 toxin； recombinant antibodies； ELISA； noncompetitive； Fab；scFv；alkaline phosphatase；fusion protein；cereal grains
作者：Arola, HO；Tullila, A；Nathanail, AV；Nevanen, TK
作者单位：VTT Tech Res Ctr Finland
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：4
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