中文摘要：作者描述了一种检测黄曲霉毒素B1(AFB1)的流式细胞仪免疫分析法。研究发现，多重荧光UCNMMs（上转换荧光纳米晶编码的磁性微球），其颜色依赖于NaYF4基质晶格中的掺杂剂种类（镱，铒，铥，锰），是一个很有前途的分析霉菌毒素和其他物质的工具。
外文摘要：The authors describe a flow cytometric immunoassay for aflatoxin B1 (AFB1). It has three distinct features: (a) Magnetic microspheres encoded with upconverting nanocrystals (UCNMMs) are used as fluorescent labels. These have the advantage of non-overlapping spectra and lacking crosstalk between the encoding signal and reporter signal via the low-energy near-infrared (NIR) light excitation; (b) phycoerythrin-labeled secondary antibodies are used to amplify the reporter signal; (c) The use of magnetic nanoparticles facilitates the rapid separation and specific purification of the analyte (AFB1). This assay has a detection limit of 9 pg·mL−1 and a broad working range for AFB1, requires a 50 μL sample only, and can be completed within 2 h with good accuracy and high reproducibility. It is perceived that such multifluorescent UCNMMs, whose color depends on the kind of dopants (Yb, Er, Tm, Mn) in the NaYF4 host lattice, represent a promising tool for the analysis of mycotoxins and other analytes.
外文关键词：Mycotoxin；Upconversion nanocrystals；Magnetic nanoparticles；Mesoporous polystyrene microspheres；Self-healing encapsulation strategy；Flow cytometric analysis；Indirect competitive immunoassay
作者：Zhang, Y；Liao, ZY；Liu, YJ；Wan, YJ；Chang, J；Wang, HJ
作者单位：Tianjin Univ
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2017
出版刊次：5
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