中文摘要：从巴基斯坦信德省因在巴昆瑞镇收集了不同品种的红辣椒。该镇是亚洲红辣椒生产中心。共收集了6个品种的69个样品，分析了黄曲霉毒素及黄曲霉的存在情况，探索抗感种质的潜力。本研究首次探索到两个不易感黄曲霉及黄曲霉毒素污染的潜在品种，它们更适宜栽培，从而促进巴基斯坦的红辣椒生产。
外文摘要：Various cultivars of red chilli were collected from a small town named Kunri, located in the province Sindh, Pakistan. This town is a hub of red chilli production in Asia. A total of 69 samples belonging to 6 cultivars were obtained and analysed for the occurrence of aflatoxins and Aspergillus flavus, to explore the potential of resistant and susceptible germplasm. Aflatoxins were detected by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC), while A. flavus was isolated and identified using agar plate, blotter paper, deep freezing and dilution techniques. Molecular characterization using internal transcribed spacer (ITS) 1/4 and A. flavus specific FL1-F/R primers confirmed the identity of A. flavus. The data revealed that 67 and 75% samples contaminated with aflatoxin B1 (AFB1) and with A. flavus, respectively. A highly susceptible chilli cultivar was ‘Nagina’, showing 78.8% frequency of total aflatoxins (1.2–600 μg/kg) and a mean of 87.7 μg/kg for AFB1 and 121.9 μg/kg for total aflatoxins. A. flavus was detected with 93% frequency and 2.14 × 104 colony forming units. In contrast, cultivars ‘Kunri’ and ‘Drooping Type’ were found to be resistant, with low levels of aflatoxins and fungal counts. The study was conducted for the first time to explore two potential cultivars that were less susceptible towards A. flavus and aflatoxin contamination. These cultivars could be preferably cultivated and thereby boost Pakistan’s chilli production.
外文关键词：Capsicum annuum；Aspergillus flavus；Aflatoxin；Resistance；Susceptible；Cultivars
作者：Akhund, S；Akram, A；Hanif, NQ；Qureshi, R；Naz, F；Nayyar, BG
作者单位：Pir Mehr Ali Shah Arid Agr Univ
期刊名称：MYCOTOXIN RESEARCH
期刊影响因子：2.0
出版年份：2017
出版刊次：2
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