中文摘要：环匹阿尼酸(α-CPA)是一种震颤真菌毒素，通常由某些曲霉，特别是黄曲霉产生，而黄曲霉更广为人知的是产生黄曲霉毒素。尽管α-CPA与黄曲霉毒素可能共同发生，却尚未广泛研发检测CPA的免疫分析法。本研究报道了几种α-CPA的单克隆抗体(mAbs)的研发及评估。结果表明研发的抗体及CI-ELISA将是进一步评估玉米中这种霉菌毒素普遍存在的有用工具。
外文摘要：Cyclopiazonic acid (α-CPA) is a tremorgenic mycotoxin that is commonly produced by certain species of the aspergilli, in particular Aspergillus flavus, which is more widely known for production of the aflatoxins. Despite the fact that α-CPA may co-occur with aflatoxins, immunoassay-based methods for monitoring for CPA have not been widely developed. We report the development and evaluation of several monoclonal antibodies (mAbs) for α-CPA. Two mAbs in particular were very sensitive, with IC50s of 1.1 and 1 ng/mL (clones 1418 and 1231, respectively). Tolerances to aqueous methanol or acetonitrile were good, which permitted the development of an antigen-immobilized competitive enzyme-linked immunosorbent assay (CI-ELISA) for detection of CPA in maize. Spiked or naturally contaminated maize, extracted with aqueous methanol, was diluted with buffer for analysis. The working range for the assay (IC20 to IC80) was from 5 to 28 μg/kg. Recoveries from maize spiked over the range from 2 to 50 μg/kg averaged 88.6 ± 12.6%. Twenty-eight samples of maize were tested by both the CI-ELISA and a liquid chromatography-fluorescence (LC-FLD) method. For the five samples above the limits of quantitation of both methods, CI-ELISA tended to overestimate CPA content, a difference that we speculate may be due to related metabolites or perhaps “masked” derivatives of CPA. The antibodies developed and the resulting CI-ELISA will be useful tools for further evaluation of the prevalence of this mycotoxin in maize.
外文关键词：Cyclopiazonic acid；Antibody；Immunoassay；Detection, LC-FLD；Maize
作者：Maragos, CM；Sieve, KK；Bobell, J
作者单位：ARS
期刊名称：MYCOTOXIN RESEARCH
期刊影响因子：2.0
出版年份：2017
出版刊次：2
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