中文摘要:对小麦中出现的影响经济和公共健康的脱氧雪腐镰刀菌烯醇(DON)毒性的关注,使得人们需要依据可用设备和获得发病率所需的速度,寻找合适的、测定DON污染程度的检测方法。本研究目的在于比较两种可用的DON定量分析术与参照技术用于食品工业的性能。研究表明两种方法对定量分析小麦粉样品中DON水平均有效。本研究旨在协助生产者选择合适的工具用于分析和考虑现有的设备。
外文摘要:The concern regarding toxicity from the presence of deoxynivalenol (DON) in wheat that affects both economy and public health leads to the need to find appropriate detection methods for determining the degree of DON contamination in terms of the equipment available and the speed required for obtaining the incidence. The objective of this study was to compare the performance of two alternative analytical techniques for DON quantification for use in the food industry with a reference technique. Samples of wheat and the commercial by-products were analysed by high-performance liquid chromatography (HPLC) with an ultraviolet detector as the reference method and the results compared with those obtained from a rapid lateral-flow immunochromatographic device (Reveal Q+) and of a Fourier-transform-infrared (FTIR) spectroscopy technique. Pearson's correlation coefficient between the HPLC and Reveal-Q+ data (0.45), although significant (P<0.0003), was lower than that obtained between HPLC and the FTIR method (0.94, P<0.0001). Both methods were considered efficient in quantifying DON levels in wheat-flour samples. This study was aimed at assisting the producers in choosing an appropriate tool for the purpose of analysis and upon consideration of the available equipment.
外文关键词:deoxynivalenol;wheat;high-performance liquid chromatography;Fourier-transform infrared spectroscopy;lateral-flow immunochromatography
作者:Astoreca, A.;Ortega, L.;Figoli, C.等
作者单位:National University of La Plata
期刊名称:WORLD MYCOTOXIN JOURNAL
期刊影响因子:2.091
出版年份:2017
出版刊次:2
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