中文摘要：霉菌毒素是由丝状真菌产生的次生代谢物，能在田间以及收获、运输、加工或储存期间污染农作物。玉米赤霉烯酮（ZEN），一种非甾体类雌激素霉菌毒素，由镰刀菌产生，已证明与牲畜的生殖障碍相关，并以更低的剂量引起人体重高雌激素综合征。因此，净化食品及饲料中的ZEN是非常重要的问题。本研究将编码ZHD101，一种由粉红粘帚霉IFO 7063产生的ZEN 降解酶的基因克隆到大肠杆菌-乳杆菌穿梭载体pNZ3004中，并通过电穿孔将合成的质粒pNZ-zhd101导入从肉鸡胃肠道分离的益生菌，罗伊乳杆菌Pg4中。转化后的罗伊乳杆菌pNZ-zhd101菌株获得了降解ZEN的能力。据悉，本研究报道的肠道乳杆菌成功表达ZEN降解酶尚属首次。
外文摘要：Background: Mycotoxins are secondary metabolites produced by filamentous fungi that can contaminate agricultural crops in the field as well as during harvest, transportation, processing, or storage. Zearalenone (ZEN), a nonsteroidal estrogenic mycotoxin, produced by Fusarium species, has been shown to be associated with reproductive disorders in farm animals and to a lesser extent in hyperoestrogenic syndromes in humans. Thus, the decontamination of ZEN in foods and feeds is an important issue. Results: In this study, the gene encoding ZHD101, a ZEN-degrading enzyme produced by Clonostachys rosea IFO 7063, was cloned into an Escherichia coli-Lactobacillus shuttle vector, pNZ3004, and the resultant plasmid pNZ-zhd101 was then introduced via electroporation into Lactobacillus reuteri Pg4, a probiotic strain isolated from the gastrointestinal tract of broilers. The transformed strain L. reuteri pNZ-zhd101 acquired the capacity to degrade ZEN. In addition, the production of recombinant ZHD101 did not affect cell growth, acid and bile salt tolerance, and had only a minor effect on the adhesion ability of L. reuteri pNZ-zhd101. Conclusions: To the best of our knowledge, this is the first report of successful expression of a ZEN-degrading enzyme by intestinal lactobacilli.
外文关键词：Zearalenone；Lactonohydrolase；Lactobacillus reuteri；Probiotics
作者：Yang, WC；Hsu, TC；Cheng, KC；Liu, JR
作者单位：Natl Taiwan Univ
期刊名称：MICROBIAL CELL FACTORIES
期刊影响因子：3.744
出版年份：2017
出版刊次：4
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