中文摘要：为得到直径约15纳米的天然磁小体，对一种普通淡水趋磁细菌 (MTB)进行了驯化并大量繁殖。研究发现主要由氧化铁和硫化铁组成的磁小体具有很好的磁性特征。此外，本研究通过化学键结合AFB1多克隆抗体与天然磁小体研发的磁小体-AFB1抗体免疫磁性探针，吸附植物油中AFB1毒素的能力是传统Fe3O4磁性纳米颗粒-AFB1抗体探针的28倍。用它处理人工AFB1污染的商品植物油15分钟，表现出高达93.7%的回收率。通过圆二色（CD）光谱测量，阐明了磁小体探针的机理，提出了基于免疫磁性分离技术，高效控制液体食品中黄曲霉毒素污染的实用方法。
外文摘要：Here, a magnetotactic bacterium (MTB) existing in freshwaters was acclimated and mass propagations for producing natural magnetosomes with diameter about 15 nm at ordinary ambient. It was found that the magnetosomes which are mainly composed of iron oxide and iron sulfide hold pretty well magnetic features. Furthermore, the developed magnetosomes-AFB 1 antibody immunomagentic probes through chemical bonding AFB 1 polyclonal antibody onto the natural magnetosomes maintain 28 fold capability on collection of AFB 1 toxin in vegetable oil compared to conventional Fe 3 O 4 magnetic nanoparticle-AFB 1 antibody probes. Recovery ratio up to 93.7% from commercial vegetable oil with artificial AFB 1 contamination during 15 min collection were demonstrated. Through circular dichroism (CD) spectral measurements, the mechanism of magnetosomes probe was interpreted for proposing the practical way with high-performance on controlling the aflatoxins contamination in liquid foods basing on immunomagentic separation technique.
外文关键词：AFB1；Magnetotactic bacteria；Magnetosomes；Elimination；Mycotoxin
作者：Pi, FW；Sun, JD；Liu, WW；Sun, XL；Zhang, YZ
作者单位：Jiangnan Univ
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2017
出版刊次：10
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