中文摘要：本文以适体荧光信号恢复为基础，设计了一种用于谷物样品中多种真菌毒素的新型高通量光子晶体微球（PHCM）悬浮阵列。研究表明该系统为并行筛选小分子提供了一种新的基于高通量适体的悬浮阵列。
外文摘要：We design a novel high throughput photonic crystal microsphere (PHCM) suspension array for multiplex mycotoxins in cereal samples based on the aptamer fluorescence signal recovery. The hybridization duplex strand DNA from mycotoxin aptamer and anti-aptamer respectively labeled with fluorescence dye and quencher was immobilized on the carboxylated surfaces of PHCMs. When the corresponding mycotoxin targets bind to their aptamers, the fluorescence recovery signal intensity of PHCMs reported the concentration of mycotoxins. The different kinds of mycotoxins were distinguished by the structure colors of PHCMs. The fluorescence signal intensity on the PHCMs is nearly higher 100 times than that of solid glass beads. The detection system presents an ultrasensitive, high selectivity, and small volume reagent screening for multiplex mycotoxins with a dynamic linear detection range of 0.1 pg/mL–0.1 ng/mL for AFB1/OTA and 0.1 ng/mL–10 ng/mL for FB1 and a limit of detection (LOD) of 15.96 fg/mL, 3.96 fg/mL and 11.04 pg/mL for AFB1, OTA and FB1, respectively. The recovery rates in spiked cereal samples were well consistent with that of the traditional ELISA. The designed system provides a new high throughput aptamer-based suspension array for small molecule screening in parallel.
外文关键词：Aptamer；Photonic crystal microsphere；Suspension array；Multiplex mycotoxin screening；Fluorescence signal recovery
作者：Yang, Y；Li, W；Shen, P；Liu, R；Li, YW；Xu, JJ；Zheng, Q；Zhang, Y；Li,JL；Zheng, TS
作者单位：Nanjing Normal Univ
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL
期刊影响因子：4.758
出版年份：2017
出版刊次：9
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