中文摘要：烟曲霉毒素C和震颤真菌毒素是由耐热费氏新萨托菌产生的霉菌毒素。用选择性和灵敏的分析方法简便快速地监测这些毒素，对于确保果实采后品质、食品安全以及人类健康至关重要。本文研制了一种六分钟液相色谱串联质谱法(LC /MS/ MS)，用于测定马铃薯葡萄糖肉汤（PDB）、草莓汁、草莓果实和土壤中烟曲霉毒素C和震颤真菌毒素。并用新研发的LC / MS / MS法对环境中45株新萨托菌：5株作为参考的已知菌株和40株新分离并鉴定的菌株产生的霉菌毒素进行了检测。
外文摘要：Fumitremorgin C and verruculogen are mycotoxins produced by heat-resistant Neosartorya fischeri. Easy and fast monitoring of their production by selective and sensitive analytical methods is crucial for ensuring postharvest quality of fruit, food safety, and thus human health and well-being. A six-minute liquid chromatography tandem mass spectrometry method (LC/MS/MS) was developed for quantification of fumitremorgin C and verruculogen in potato dextrose broth (PDB), strawberry juice, strawberry fruit, and soil. Mycotoxins were extracted from all media with the QuEChERS (Quick Easy Cheap Effective Rugged Safe) method. The lower limit of quantification (LLOQ) of the method for both mycotoxins was 1 μg L−1 for PDB and strawberry juice and 1 μg kg−1 for strawberry fruit and soil. Collision-induced dissociation of protonated fumitremorgin C and verruculogen ions was also examined by high-resolution mass spectrometry to assist researchers in non-targeted screening for mycotoxins and in identification of these two toxins. The developed LC/MS/MS method was applied to examine mycotoxin production by 45 environmental Neosartorya strains: 5 well-known strains, included in the study as reference strains, and 40 strains that were newly isolated and identified by us.
外文关键词：Heat resistant fungi；Mycotoxin；Fruit contamination；Neosartorya；Liquid chromatography tandem mass spectrometry method
作者：Fornal, E；Parfieniuk, E；Czeczko, R；Bilinska-Wielgus, N；Frac, M
作者单位：Med Univ Lublin
期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY
期刊影响因子：2.618
出版年份：2017
出版刊次：9
点击下载：用快速简便的液相色谱-质谱法测定真菌毒素：烟曲霉毒素C和震颤真菌毒素，评估采后果品的安全性