中文摘要：玉米赤霉烯酮（ZEN）主要是由镰刀菌产生的霉菌毒素。谷物和植物受真菌污染后就会产生ZEN，出现在不同的食品、动物饲料及饮品中。因ZEN是内分泌干扰物，引起牲畜生殖紊乱及人体高雌激素综合征。尽管在很多物种中研究了ZEN的毒代动力学特性，但缺乏关于ZEN与血清白蛋白相互作用的资料。既然白蛋白通常在不同毒素的毒代动力学中起着重要的作用，那么ZEN与白蛋白的相互作用就具有很高的生物学意义。因此本文采用光谱法、超滤法及分子模拟法研究了ZEN与人血清白蛋白（HSA）的相互作用。研究表明，如果要准确地理解ZEN的毒代动力学行为，那我们就应该记住这种相互作用。
外文摘要：Zearalenone (ZEN) is a mycotoxin produced mainly by Fusarium species. Fungal contamination of cereals and plants can result in the formation of ZEN, leading to its presence in different foods, animal feeds, and drinks. Because ZEN is an endocrine disruptor, it causes reproductive disorders in farm animals and hyperoestrogenic syndromes in humans. Despite toxicokinetic properties of ZEN were studied in more species, we have no information regarding the interaction of ZEN with serum albumin. Since albumin commonly plays an important role in the toxicokinetics of different toxins, interaction of ZEN with albumin has of high biological importance. Therefore the interaction of ZEN with human serum albumin (HSA) was investigated using spectroscopic methods, ultrafiltration, and molecular modeling studies. Fluorescence spectroscopic studies demonstrate that ZEN forms complex with HSA. Binding constant (K) of ZEN-HSA complex was quantified with fluorescence quenching technique. The determined binding constant (logK = 5.1) reflects the strong interaction of ZEN with albumin suggesting the potential biological importance of ZEN-HSA complex formation. Based on the results of the investigations with site markers as well as docking studies, ZEN occupies a non-conventional binding site on HSA. Considering the above listed observations, we should keep in mind this interaction if we would like to precisely understand the toxicokinetic behavior of ZEN.
外文关键词：Zearalenone；Human serum albumin；Albumin-ligand interaction；Fluorescence spectroscopy
作者：Poor, M；Kunsagi-Mate, S；Balint, M；Hetenyi, C；Gerner, Z；Lemli, B
作者单位：Univ Pecs
期刊名称：JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
期刊影响因子：3.035
出版年份：2017
出版刊次：5
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