中文摘要：本研究描述了一种检测黄曲霉毒素B1和伏马菌素的采用蓝色沙漠玫瑰状金纳米粒子（DR-GNPs）和红色球状GNPs的多色免疫层析法（ICST）。它能同时快速检测玉米粉中的2种霉菌毒素，可见的分界水平分别为：黄曲霉毒素B1 2μg/kg及伏马菌素1000μg/kg。
外文摘要：The authors describe the preparation of desert rose-like gold nanoparticles (DR-GNPs) with a plasmon resonance band at 620 nm which gives them a blue color. They have a hydrodynamic diameter of ∼72 nm and were prepared by a seeding growth approach. The DR-GNPs were characterized by UV-vis spectroscopy, transmission electron microscopy and dynamic light scattering. These nonspherical GNPs were used as a label for the antibody in an immunochromatographic strip test (ICST). Despite their particular shape and the higher surface area compared to spherical gold nanoparticles, the DR-GNPs are useful blue labels for the GNP-based strip test. A multicolor ICST for aflatoxin B1 and fumonisins is described that employs both blue DR-GNPs and red spherical GNPs. It allows for simultaneous rapid determination of the two mycotoxins in maize flour, with visual cut-off levels of 2 μg⋅kg-1 for aflatoxin B1 and of 1000 μg⋅kg-1 for fumonisins.
外文关键词：Non-spherical gold nanoparticles；Labels for immunoassay；Lateral flow assay；Aflatoxin B1；Fumonisins；Maize
作者：Di Nardo, F；Baggiani, C；Giovannoli, C；Spano, G；Anfossi, L
作者单位：Univ Turin
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2017
出版刊次：5
点击下载：用基于纳米金的多色免疫层析法检测黄曲霉毒素和伏马菌素