中文摘要：本研究描述了一种快速萃取方法的发展和谷物样品（小麦和玉米粉）中频繁共现的三种霉菌毒素，脱氧雪腐镰刀菌烯醇、伏马菌素B1和赭曲霉毒素A的免疫化学检测。本方法是用于快速检测商品谷物和饲料样品中FB1、DON和OTA的高通量精确方法。
外文摘要：This work describes the development of a rapid method for the extraction and the immunochemical determination of three mycotoxins, deoxynivalenol, fumonisin B1, and ochratoxin A, which are frequently found together naturally in cereal samples (wheat and corn flours). The 3 mycotoxins were extracted simultaneously in dichloromethane, and then three parallel spectrophotometric enzyme immunoassays were carried out in conventional microtiter plates using magnetic beads. Three specific monoclonal mouse antibodies for the three mycotoxins were immobilized in an oriented way using protein G functionalized magnetic particles. The m agnetic immunoassay schemes decrease the incubation times, improve greatly the efficiency of the separation and washing steps, and also allow the easy elimination of interfering matrix components from the extracted samples. The method has limits of detection of 5.0 +/- 1.4 ng mL(-1) for FB1, 4.3 +/- 1.8 ng mL(-1) for DON, and 0.1 +/- 0.05 ng mL(-1) for OTA (n - 5), and the relative standard deviations of the determinations of the three mycotoxins are less than about 10% RSD. The method allows us to measure concentrations of these mycotoxins well below the limits of the European Union legislation in cereal samples. The developed multiplex magnetic particle-based enzyme immunoassay (mpEIA) method was validated with certified reference materials (wheat and maize flour samples) and three official AOAC (Association of Analytical Chemists) chromatographic analytical methods for each of the three mycotoxins. This method is high-throughput and accurate for the rapid determination of FB1, DON, and OTA in commercial cereal and feedstuff samples.
外文关键词：IMMUNOCHEMICAL METHODS；FOODSTUFFS；BIOSENSORS；TOXINS
作者：Vidal, J. C.；Bertolin, J. R.；Ezquerra, A.等
作者单位：University of Zaragoza
期刊名称：ANALYTICAL METHODS
期刊影响因子：1.915
出版年份：2017
出版刊次：24
点击下载：快速同步萃取和磁性粒子酶免疫分析用于同步检测谷物样品中的赭曲霉毒素A、伏马菌素B1和脱氧雪腐镰刀菌烯醇毒素