中文摘要：本研究利用液相色谱-质谱联用（LC-MS）分析了2014-2015年之间捷克不同地区随机收集的244份麦穗样品中的霉菌毒素，并用PCR检测鉴定了引起赤霉病的镰刀菌种类。
外文摘要：Mycotoxin content in 244 samples of wheat ears randomly collected during 2014 and 2015 from various localities in the Czech Republic was analysed using liquid chromatography coupled to mass spectrometry (LC-MS). Mean mycotoxin concentration in 2014 was highest for deoxynivalenol (DON; 760 mu g/kg), followed by zearalenone (ZEA; 115 mu g/kg), 3-acetyldeoxynivalenol (3-ADON; 88 mu g/kg), deoxynivalenol-3-glucoside (83 mu g/kg), and enniatins (ENNs; 102 mu g/kg). In 2015, DON (66 mu g/kg) also had the highest concentration level, followed by ENNs (35 mu g/kg), nivalenol (2 mu g/kg), and beauvericin (2 mu g/kg). The maximum limit for DON in the European Union (1,250 mu g/kg) was exceeded in 2% of samples, and the maximum limit for ZEA (100 mu g/kg) was exceeded in 0.8% of samples. Fusarium species causing head blight were identified using PCR assays. During 2014-2015, Fusarium poae considerably dominated (48.7% average value of occurrence in the samples). Other species were detected in much lower frequencies in both years: Fusarium graminearum (average frequency of occurrence 13.7%), Fusarium avenaceum (11.9%), Fusarium culmorum (4.2%), and Fusarium equiseti (2.9%). Fusarium langsethiae was identified only in 2015, at a frequency of 10.2%, and Fusarium sporotrichioides was present only sporadically in 2014.
外文关键词：Fusarium；deoxynivalenol；enniatins；beauvericin；zearalenone
作者：Sumikova, T.；Chrpova, J.；Dzuman, Z.；等
作者单位：Czech Crop Research Institute
期刊名称：WORLD MYCOTOXIN JOURNAL
期刊影响因子：2.091
出版年份：2017
出版刊次：2
点击下载：捷克小麦中霉菌毒素含量及其与镰刀菌病原体变化模式的联系