中文摘要：霉菌和霉菌毒素暴露不仅会导致呼吸道疾病的发生，还会影响眼表。已发表的研究中很少涉及霉菌毒素暴露对眼细胞影响的评估。本研究调查曲霉菌分泌的两种霉菌毒素，黄曲霉毒素B1（AFB1）和胶霉毒素对人角膜上皮（HCE）细胞生物活性的影响。结果表明，由环境因素，如真菌毒素引起的低而持续的炎症可导致刺激性和致敏性，并可能引起过敏反应，这反过来又可能导致粘膜高反应。
外文摘要：Exposure to molds and mycotoxins not only contributes to the onset of respiratory disease, it also affects the ocular surface. Very few published studies concern the evaluation of the effect of mycotoxin exposure on ocular cells. The present study investigates the effects of aflatoxin B1 (AFB1) and gliotoxin, two mycotoxins secreted by Aspergillus molds, on the biological activity of the human corneal epithelial (HCE) cells. After 24, 48, and 72 h of exposure, cellular viability and inflammatory response were assessed. Both endpoint cell viability colorimetric assays and continuous cell impedance measurements, providing noninvasive real-time assessment of the effect on cells, were performed. Cytokine gene expression and interleukin-8 release were quantified. Gliotoxin appeared more cytotoxic than AFB1 but, at the same time, led to a lower increase of the inflammatory response reflecting its immunosuppressive properties. Real-time cell impedance measurement showed a distinct profile of cytotoxicity for both mycotoxins. HCE cells appeared to be a well-suited in vitro model to study ocular surface reactivity following biological contaminant exposure. Low, but persistent inflammation, caused by environmental factors, such as fungal toxins, leads to irritation and sensitization, and could be responsible for allergic manifestations which, in turn, could lead to mucosal hyper-reactivity.
外文关键词：mycotoxin；aflatoxin B1；gliotoxin；in vitro；ocular surface；inflammatory response；cellular impedance
作者：Bossou, YM；Serssar, Y；Allou, A；Vitry, S；Momas, I；Seta, N；Menotti,J；Achard, S
作者单位：Paris Descartes Univ
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：9
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