中文摘要：本研究从花生中分离出20株黑曲霉，研究发现其中14株能够通过共培养完全抑制AFB1产生。研究还发现不产毒的黄曲霉JZ2和 GZ15能有效降解AFB1。
外文摘要：Twenty Aspergillus niger strains were isolated from peanuts and 14 strains were able to completely inhibit AFB1 production with co-cultivation. By using a Spin-X centrifuge system, it was confirmed that there are some soluble signal molecules or antibiotics involved in the inhibition by A. niger, although they are absent during the initial 24h of A. flavus growth when it is sensitive to inhibition. In A. flavus, 19 of 20 aflatoxin biosynthetic genes were down-regulated by A. niger. Importantly, the expression of aflS was significantly down-regulated, resulting in a reduction of AflS/AflR ratio. The results suggest that A. niger could directly inhibit AFB1 biosynthesis through reducing the abundance of aflS to aflR mRNAs. Interestingly, atoxigenic A. flavus JZ2 and GZ15 effectively degrade AFB1. Two new metabolites were identified and the key toxic lactone and furofuran rings both were destroyed and hydrogenated, meaning that lactonase and reductase might be involved in the degradation process.
外文关键词：Aspergillus flavus；Aspergillus niger；Aflatoxin；Inhibition；Degradation
作者：Xing, FG；Wang, LM；Liu, X；Selvaraj, JN；Wang, Y；Zhao, YJ；Liu, Y
作者单位：Chinese Acad Agr Sci
期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
期刊影响因子：3.445
出版年份：2017
出版刊次：9
点击下载：黑曲霉通过下调主要生物合成基因的表达对黄曲霉中黄曲霉毒素B1的抑制及无毒黄曲霉对AFB1的降解