中文摘要：本研究用亚甲基蓝（MB）氧化还原探针标记的适体作信号片段，并用功能化氧化石墨烯（FGO）作信号放大平台，开发了电化学传感器。通过检测样品中的AFB1筛选酒精饮料，来检验本传感器，获得了良好的回收率。
外文摘要：In this work, we developed an electrochemical aptasensor by using methylene blue (MB) redox probe labeled aptamer as a signaling fragment and functional graphene oxide (FGO) as the signal-enlarging platform. The role of functionalized graphene oxide was not mere to serve as a covalent immobilization support for aptamer sequences, but its catalytic signal amplification behavior towards methylene blue was demonstrated for the first time in the present work. The functionalized graphene oxide was cast on screen-printed carbon electrodes (SPCE), and then the MB-tagged aptamer was covalently immobilized on SPCE by using hexamethylenediamine (HMDA) as a spacer via carbodiimide amide-bonding chemistry. Aflatoxin B1 (AFB1) analyte molecule detection was accomplished by the aptamer conjugated redox probe, which undergoes/involves a conformational change in the complex structure of aptamer consequent to AFB1 binding. The proposed assay permitted to detect AFB1 in the linear range of 0.05–6.0 ng mL−1 with a very low limit of detection (LOD) (0.05 ng mL−1). The present principle aptasensor was tested to screen the alcoholic beverage samples for AFB1 detection and good recovery values were obtained.
外文关键词：Aptamer；Aflatoxin B1；DPV；Alcoholic beverages；Graphene；Methylene blue
作者：Goud, KY；Hayat, A；Catanante, G；Satyanarayana, M；Gobi, KV；Marty, JL
作者单位：Univ Perpignan
期刊名称：ELECTROCHIMICA ACTA
期刊影响因子：4.803
出版年份：2017
出版刊次：8
点击下载：基于功能化氧化石墨烯辅助亚甲基蓝电催化信号放大的电化学传感器，用于检测黄曲霉毒素B1