中文摘要：据报道，在动物和人体中，赭曲霉毒素A（OTA）是强效的肾毒素和免疫毒素。然而，迄今为止，OTA的毒性机制尚未明确。本文在PK15细胞和猪原代脾细胞中研究了OTA的毒性及其机制。结果表明，OTA通过p38信号通路诱导PK15细胞的肾毒性，通过ERK信号通路诱导猪原代脾细胞的免疫毒性。
外文摘要：Ochratoxin A (OTA) is reported to be a potent nephrotoxin and immunotoxin in animals and humans. However, the mechanisms underlying OTA toxicity have not been clearly determined until now. Toxicity of OTA and its mechanism was investigated in PK15 cells and in porcine primary splenocytes. The results showed that OTA at 2.0-8.0 μg/mL for 24 h induced cytotoxicity and apoptosis in a dose-dependent manner in PK 15 cells. OTA at 0.5-4.0 μg/mL for 24 h induced cytotoxicity and apoptosis in a dose-dependent manner in porcine primary splenocytes. In addition, OTA induced p38 and ERK1/2 phosphorylation both in PK15 cells and porcine primary splenocytes. Knock-down of p38 instead of ERK by their specific siRNA significantly eliminated the nephrotoxicity induced by OTA. Contrary, knock-down of ERK1/2 instead of p38 by their specific siRNA significantly eliminated the immunotoxicity induced by OTA. The observed effects indicate that OTA induced nephrotoxicity by p38 signaling pathway in PK15 cells and immunotoxicity by ERK signaling pathway in porcine primary splenocytes.
外文关键词：Ochratoxin A；Nephrotoxicity；Immunotoxicity；MAPK signaling pathway；PK15 cells；Porcine primary splenocytes
作者：Gan, F；Zhou, YJ；Hou, LL；Qian, G；Chen, XX；Huang, KH
作者单位：Nanjing Agr Univ
期刊名称：CHEMOSPHERE
期刊影响因子：3.698
出版年份：2017
出版刊次：9
点击下载：赭曲霉毒素A在PK15细胞和猪原代脾细胞中通过不同的MAPK信号通路诱导肾毒性和免疫毒性