中文摘要：本研究开发了一种简便的细胞电化学生物传感器，评估脱氧雪腐镰刀菌烯醇（DON）、玉米赤霉烯酮（ZEN）和黄曲霉毒素B1（AFB1）对Hep G2细胞的单独及联合毒性。研究表明，相比于最常用的细胞毒性评价方法，该方法更简单、方便、灵敏，并具有较快的响应速度。
外文摘要：A simple and convenient cell-based electrochemical biosensor was developed to assess the individual and combined toxicity of deoxynivalenol (DON), zearalenone (ZEN), and Aflatoxin B1 (AFB1) on Hep G2 cells. The sensor was modified in succession with AuNPs (gold nanoparticles), cysteamine, and laminin. The cells interacting with laminin formed tight cell-to-electrode contacts, and collagen was used to maintain cell adhesion and viability. Electrochemical impedance spectroscopy (EIS) was developed to evaluate mycotoxin toxicity. Experimental results show that DON, ZEN, and AFB1 caused a significant decrease in cell viability in a dose dependent manner. The EIS value decreased with concentrations of DON, ZEN, and AFB1 in the range of 0.01-20, 0.1-50, and 0.1-3.5μg/mL, and IC50 obtained using the developed method was 48.5, 59.0, and 3.10μg/mL, respectively. A synergistic effect was observed between DON and ZEN, an additive effect was observed between DON and AFB1, and an antagonism effect was found in the binary mixtures of ZEN and AFB1 and ternary mixtures. These results were confirmed via CCK-8 assay. Utilizing SEM, we found that cells treated with mycotoxins caused significant changes in cell morphology, thus lessening cell adsorption and impedance reduction. Biological assay indicated that EIS patterns correlated with [Ca2+]i concentrations and apoptosis and necrotic cells ratios, thus effecting electrochemical signals. This method is simpler, more convenient, sensitive, and has a quicker response rate than most conventional cytotoxicity evaluation methods.
外文关键词：DON；ZEN；AFB(1)；Combination；Toxicity；Electrochemistry
作者：Xia, SA；Zhu, P；Pi, FW；Zhang, YZ；Li, Y；Wang, JS；Sun, XL
作者单位：Jiangnan Univ
期刊名称：BIOSENSORS & BIOELECTRONICS
期刊影响因子：7.476
出版年份：2017
出版刊次：11
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