中文摘要：黄曲霉毒素B1（AFB1）是全球最丰富、最致癌的食品污染霉菌毒素。本研究提出了一种检测AFB1的表面增强拉曼散射（SERS）传感策略。结果表明当AFB1的浓度为1×10−6 to 1 ng/mL时，具有良好的线性关系；检测限（LOD）为0.4 fg/mL。该传感器还被用于检测加标花生样品中AFB1的含量，回收率在89–121%之间。
外文摘要：Aflatoxin B1 (AFB1) is one of the most abundant and carcinogenic food-contaminating mycotoxins around the world. In this study, we proposed a surface enhanced Raman scattering (SERS) sensing strategy for the determination of AFB1. An aptamer for AFB1 partially hybridized with complementary-DNA, which was released after the recognition of AFB1 and immediately hybridized with hairpin DNA on the surface of sputtering Au film. Exonuclease III hydrolyzed the double-stranded DNA, leaving short single-stranded DNA on the Au surface and releasing complementary-DNA for next ring opening and digestion. SERS tag was captured on Au surface by DNA hybridization. Agarose gel electrophoresis and dynamic light scattering showed that SERS tag was successfully prepared. The detection principle was validated by electrochemical impedance spectroscopy and SERS at each step. High sensitivity and good selectivity for AFB1 detection were observed. The results showed that there was a good linear relation when the AFB1 concentration was from 1×10−6 to 1 ng/mL, and the limit of detection (LOD) was 0.4 fg/mL. This sensor was also applied for quantifying AFB1 levels in spiked peanuts samples, the recoveries was in the range of 89–121%.
外文关键词：Aflatoxin B-1；Aptamer；Exonuclease III；Recycling amplification；Surface enhanced Raman scattering
作者：Li, Q；Lu, ZC；Tan, XC；Xiao, XY；Wang, P；Wu, L；Shao, K；Yin, WM；Han, HY
作者单位：Huazhong Agr Univ
期刊名称：BIOSENSORS & BIOELECTRONICS
期刊影响因子：7.476
出版年份：2017
出版刊次：11
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