中文摘要：黄曲霉和寄生曲霉是黄曲霉毒素(AFs)生产者，能在干腌火腿的成熟过程中生长。本研究评估了不同水活度(aw)及温度对参与AFs生物合成的三个基因的时间相对表达的影响，以及它们与AFs在干腌火腿培养基上产生的关系。研究表明早期发现调控基因的表达可以很好地反映干腌火腿成熟过程中可能发生的AFS污染。
外文摘要：Aspergillus flavus and Aspergillus parasiticus are mould species producers of aflatoxins (AFs) and may grow on dry-cured ham during the ripening process. In this study, the influence of different water activity (aw) and temperatures on the temporal relative expression of three genes involved in AFs biosynthesis and their relationship with AFs production on dry-cured ham-based medium were evaluated. In general, the regulatory aflR and aflS genes showed similar expression patterns, and the expression of the structural aflP gene was much higher than that obtained for aflR and aflS genes. Regarding A. flavus, a decrease of aw regardless of temperature caused an increase of the expression of the regulatory aflR and aflS genes. Concerning A. parasiticus, the highest and lowest expression values of the regulatory aflR and aflS genes were found at 0.95 aw and 0.85 aw, respectively. The expression of the structural aflP gene of both species was stimulated at low temperature and aw. The PCA analysis indicated that both toxigenic species showed a strong correlation between the relative expression of the aflR and aflS genes and the concentration of AFs under conditions which simulate dry-cured ham ripening. This suggests that an early detection of the expression of regulatory genes can be a good indicator of possible AFs contamination of dry-cured ham through ripening.
外文关键词：Aspergillus flavus；Aspergillus parasiticus；Aflatoxins；Dry-cured ham；Gene expression
作者：Peromingo, B；Rodriguez, M；Delgado, J；Andrade, MJ；Rodriguez, A
作者单位：Univ Extremadura
期刊名称：FOOD MICROBIOLOGY
期刊影响因子：3.682
出版年份：2017
出版刊次：10
点击下载：基因表达是干腌火腿中黄曲霉毒素污染的良好指示剂