中文摘要：许多中等致病性的镰刀菌产生恩链孢菌素，一族具有杀虫及植物毒素活性的环六缩肽类。本研究研发了一种结合产品离子和中性损耗滤波的利用逆相C8 UHPLC色谱柱的半靶向LC-MS/MS法，检测样品中的恩链孢菌素及相关结构的化合物。
外文摘要：Several moderately pathogenic Fusarium species produce enniatins, a family of cyclohexadepsipeptides with insecticidal and phytotoxic activities. A semi-targeted LC-MS/MS approach utilizing reversed-phase C8 UHPLC column chromatography that combines both product ion and neutral loss filtering was developed to detect enniatins and structurally-related compounds in a sample. Using this methodology, resolution of all major enniatins was achieved with a 6-min LC run. From extracts of F. avenaceum, F. acuminatum and F. subglutinans, 23 enniatins and 16 previously unreported bassianolide-like cyclooctadepsipeptides were detected. Enniatins B, B1, A1 and A were the most frequently detected enniatins from silage and inoculated maize. The newly discovered bassianolide compounds were also detected, in lesser amounts on inoculated maize and in naturally contaminated silage.
外文关键词：LC-MS/MS；Emerging mycotoxins；Product ion filtering；Neutral loss filtering；Enniatin；Bassianolide
作者：Renaud, JB；Kelman, MJ；McMullin, DR；Yeung, KKC；Sumarah, MW
作者单位：Agr & Agri Food Canada
期刊名称：JOURNAL OF CHROMATOGRAPHY A
期刊影响因子：3.926
出版年份：2017
出版刊次：7
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